亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Cryo-EM structures of infectious bursal disease viruses with different virulences provide insights into their assembly and invasion

传染性法氏囊病 病毒学 病毒 生物 毒力 衣壳 拉伤 遗传学 基因 解剖
作者
Keyan Bao,Xiaole Qi,Yan Li,Minqing Gong,Xiaomei Wang,Ping Zhu
出处
期刊:Science Bulletin [Elsevier BV]
卷期号:67 (6): 646-654 被引量:15
标识
DOI:10.1016/j.scib.2021.12.009
摘要

Infectious bursal disease virus (IBDV) causes a highly contagious immunosuppressive disease in chickens, resulting in significant economic losses. The very virulent IBDV strain (vvIBDV) causes high mortality and cannot adapt to cell culture. In contrast, attenuated strains of IBDV are nonpathogenic to chickens and can replicate in cell culture. Although the crystal structure of T = 1 subviral particles (SVP) has been reported, the structures of intact IBDV virions with different virulences remain elusive. Here, we determined the cryo-electron microscopy (cryo-EM) structures of the vvIBDV Gx strain and its attenuated IBDV strain Gt at resolutions of 3.3 Å and 3.2 Å, respectively. Compared with the structure of T = 1 SVP, IBDV contains several conserved structural elements unique to the T = 13 virion. Notably, the N-terminus of VP2, which is disordered in the SVP, interacts with the SF strand of VP2 from its neighboring trimer, completing the β-sheet of the S domain. This interaction helps to form a contact network by tethering the adjacent VP2 trimers and contributes to the assembly and stability of the IBDV virion. Structural comparison of the Gx and Gt strains indicates that H253 and T284 in the VP2 P domain of Gt, in contrast to Gx, form a hydrogen bond with a positively charged surface. This suggests that the combined mutations Q253H/A284T and the associated structural electrostatic features of the attenuated Gt strain may contribute to adaptation to cell culture. Furthermore, a negatively charged groove in VP2, containing an integrin binding IDA motif that is critical for virus attachment, was speculated to play a functional role in the entry of IBDV.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
董可以发布了新的文献求助10
刚刚
HCB1发布了新的文献求助10
1秒前
董可以完成签到,获得积分10
8秒前
传奇3应助科研通管家采纳,获得10
9秒前
小二郎应助科研通管家采纳,获得10
9秒前
领导范儿应助科研通管家采纳,获得10
9秒前
9秒前
Hello应助科研通管家采纳,获得10
9秒前
blue完成签到 ,获得积分10
11秒前
东风完成签到,获得积分10
12秒前
ceeray23发布了新的文献求助40
12秒前
万能图书馆应助HCB1采纳,获得10
12秒前
玩命的糖豆完成签到 ,获得积分10
19秒前
科研小白完成签到,获得积分10
20秒前
28秒前
健康的大船完成签到 ,获得积分10
29秒前
橙子完成签到 ,获得积分10
34秒前
山城小丸完成签到,获得积分10
41秒前
吴嘉俊发布了新的文献求助10
42秒前
43秒前
小丸子完成签到 ,获得积分10
44秒前
彩色橘子完成签到 ,获得积分10
45秒前
48秒前
yyf251发布了新的文献求助10
53秒前
阿金啊完成签到,获得积分10
53秒前
阿金啊发布了新的文献求助30
56秒前
cyy完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
luang完成签到,获得积分10
1分钟前
个性慕青完成签到 ,获得积分10
1分钟前
丘丘完成签到,获得积分10
1分钟前
RRRZZ完成签到 ,获得积分10
1分钟前
习习完成签到,获得积分10
1分钟前
所所应助小真白采纳,获得10
1分钟前
科研通AI2S应助内向的小脑采纳,获得10
1分钟前
mingli的tau发布了新的文献求助10
1分钟前
科研通AI5应助LJ_scholar采纳,获得10
1分钟前
1分钟前
yf完成签到,获得积分10
1分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990012
求助须知:如何正确求助?哪些是违规求助? 3532068
关于积分的说明 11256227
捐赠科研通 3270933
什么是DOI,文献DOI怎么找? 1805123
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809216