免疫沉淀
污渍
蛋白质检测
酵母
化学
分子生物学
抗体
生物化学
生物
细胞生物学
遗传学
基因
纳米技术
材料科学
作者
Erica Gerace,Danesh Moazed
出处
期刊:Elsevier eBooks
[Elsevier]
日期:2014-01-01
卷期号:: 13-26
被引量:14
标识
DOI:10.1016/b978-0-12-420119-4.00002-1
摘要
This protocol outlines a procedure for testing whether two proteins interact. A target protein will be immunoprecipitated using an antibody that recognizes it (or a tagged version of the protein). The immunoprecipitated material will be separated by SDS-PAGE and analyzed by Western blotting to assess the presence of a candidate interacting protein(s).
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