Adrenal sympathetic nerve mediated the anti-inflammatory effect of electroacupuncture at ST25 acupoint on a rat model of sepsis

电针 医学 败血症 刺激 内科学 麻醉 内分泌学 脂多糖 交感肾系统 交感神经系统 针灸科 儿茶酚胺 血压 病理 替代医学
作者
Ziyi Zhang,Xiang Cui,Kun Liu,Xinyan Gao,Qingchen Zhou,Hanqing Xi,Yingkun Zhao,Dingdan Zhang,Bing Zhu
标识
DOI:10.1101/2022.07.14.499985
摘要

Abstract Acupuncture plays a vital anti-inflammatory action on sepsis through activating autonomic nerve anti-inflammatory pathways, such as sympathoadrenal medullary pathway, but the mechanism remains unclear. This study aims to explore the optimum parameter of electroacupuncture (EA) stimulation in regulating sympathoadrenal medullary pathway and evaluate EA’s anti-inflammatory effect on sepsis. To explore the optimum parameter of EA at homosegmental acupoint on adrenal sympathetic activity, the left adrenal sympathetic nerve firing rate evoked by different intensities of single shock electrical stimulation (ES) at ST25 in healthy male Sprague-Dawley (SD) rats were evaluated by in vivo electrophysiological recording, and the levels of norepinephrine (NE) and its metabolites were also examined using mass spectrometry. To verify the role of EA at ST25 in sepsis, the rat was given intraperitoneal injection lipopolysaccharide to induce sepsis model, and survival rate, clinical score, and the level of interleukin (IL)-6, IL-1β, and IL-10 were evaluated after EA application. We observed that 3 mA is the optimal intensity on activating adrenal sympathetic nerve, which significantly elevated the level of NE in the peripheral blood. For LPS-treated rats, EA at the ST25 apparently increased the survival rate and improved the clinical score compared to the control group. Furthermore, 3 mA EA at ST25 significantly decreased pro-inflammatory cytokines IL-6 and IL-1β and upregulated anti-inflammatory cytokine IL-10 compared to the Lipopolysaccharide (LPS)-treated group. Overall, these data suggest that 3 mA is the optimal EA intensity at ST25 to activate the sympathoadrenal medullary pathway and exert an anti-inflammatory effect on sepsis. Highlights 3 mA single shock ES at ST25 drove adrenal sympathetic nerve reflexs. 3 mA EA at ST25 upregulated NE concentration in peripheral blood. EA at ST25 improved the survival rate in a rat of LPS-induced sepsis model. EA at the ST25 upregulated anti-inflammatory IL10 and downregulated pro-inflammatory IL1β and IL-6 in LPS-treated rats.
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