自催化
DNA
化学
滚动圆复制
纳米技术
DNA折纸
生物分析
组合化学
生物物理学
合理设计
催化作用
生物化学
聚合酶
色谱法
材料科学
生物
作者
Peng Yang,Hao Chen,Quanjing Zhu,Zhaopeng Chen,Zezhou Yang,Ruo Yuan,Yan Li,Wenbin Liang
出处
期刊:Talanta
[Elsevier]
日期:2022-01-10
卷期号:240: 123219-123219
被引量:6
标识
DOI:10.1016/j.talanta.2022.123219
摘要
Considering the challenges of generating simple and efficient DNA (deoxyribonucleic acid) nanomachines for sensitive bioassays and the great potential of target-induced self-cycling catalytic systems, herein, a novel autocatalytic three-dimensional (3D) DNA nanomachine was constructed based on cross-catalytic hairpin assembly on gold nanoparticles (AuNPs) to generate self-powered efficient cyclic amplification. Typically, the DNA hairpins H1, H2, H3 and H4 were immobilized onto AuNPs first. In the presence of target microRNA-203a, the 3D DNA nanomachines were triggered to activate a series of CHA (catalytic hairpin assembly) reactions. Based on the rational design of the system, the products of the CHA 1 reaction were the trigger of the CHA 2 reaction, which could trigger the CHA 1 reaction in turn, generating an efficient self-powered CHA amplification strategy without adding fuel DNA strands or protein enzymes externally and producing high-efficiency fluorescence signal amplification. More importantly, the proposed autocatalytic 3D DNA nanomachines outperformed conventional 3D DNA nanomachines combined with the single-directional cyclic amplification strategy to maximize the amplification efficiency. This strategy not only achieves high-efficiency analysis of microRNAs (microribonucleic acids) in vitro and intracellularly but also provides a new pathway for highly processive DNA nanomachines, offering a new avenue for bioanalysis and early clinical diagnosis.
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