RFLP Mapping of QTL for Fusarium Head Blight Resistance in Wheat

Crop ScienceVolume 39, Issue 3 cropsci1999.0011183X003900030032x p. 805-811 Cell Biology & Molecular Genetics RFLP Mapping of QTL for Fusarium Head Blight Resistance in Wheat B. L. Waldron, B. L. Waldron USDA-ARS Forage and Range Res. Lab., Logan, UT, 84322-6300Search for more papers by this authorB. Moreno-Sevilla, B. Moreno-Sevilla HybriTech, 407 N. Cloverdale Dr., Boise, ID, 83713Search for more papers by this authorJ. A. Anderson, Corresponding Author J. A. Anderson ander319@tc.umn.edu Dep. of Agronomy and Plant Genetics, 411 Borluag, Univ. of Minnesota, St. Paul, MN, 55108Corresponding author (ander319@tc.umn.edu).Search for more papers by this authorR. W. Stack, R. W. Stack Plant Pathology Dep., Walster Hall, North Dakota State Univ., Fargo, ND, 58105-5051Search for more papers by this authorR. C. Frohberg, R. C. Frohberg Plant Sci. Dep., Loftsgard Hall, North Dakota State Univ., Fargo, ND, 58105-5051Search for more papers by this author B. L. Waldron, B. L. Waldron USDA-ARS Forage and Range Res. Lab., Logan, UT, 84322-6300Search for more papers by this authorB. Moreno-Sevilla, B. Moreno-Sevilla HybriTech, 407 N. Cloverdale Dr., Boise, ID, 83713Search for more papers by this authorJ. A. Anderson, Corresponding Author J. A. Anderson ander319@tc.umn.edu Dep. of Agronomy and Plant Genetics, 411 Borluag, Univ. of Minnesota, St. Paul, MN, 55108Corresponding author (ander319@tc.umn.edu).Search for more papers by this authorR. W. Stack, R. W. Stack Plant Pathology Dep., Walster Hall, North Dakota State Univ., Fargo, ND, 58105-5051Search for more papers by this authorR. C. Frohberg, R. C. Frohberg Plant Sci. Dep., Loftsgard Hall, North Dakota State Univ., Fargo, ND, 58105-5051Search for more papers by this author First published: 01 May 1999 https://doi.org/10.2135/cropsci1999.0011183X003900030032xCitations: 259 Research supported by USDA-NRI grant 96-35300-3721 to J.A.A., the Millers' National Federation, North Dakota State University Research Foundation, North Dakota Agric. Exp. Stn., and USDA-ARS. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Recent epidemics of fusarium head blight (FHB), caused by Fusarium graminearum Schwabe (telomorph: Gibberella zeae), in the USA and Canada have caused severe yield and quality losses in wheat (Triticum aestivum L.). Development of resistant cultivars has been difficult because of the complex inheritance of resistance and confounding environmental effects. This study was conducted to identify and map DNA markers linked to genes associated with FHB resistance. A population of 112 F5-derived recombinant inbred (RI) wheat lines from the cross ‘Sumai 3’ (resistant)/‘Stoa’ (moderately susceptible) was evaluated in two greenhouse experiments for Type II resistance (spread within the spike). On the basis of restriction fragment length polymorphism (RFLP) marker analyses, five genomic regions were significantly (P < 0.01) associated with FHB resistance, three derived from Sumai 3 and two from Stoa. Regions on Chromosomes 3BS (from Sumai 3) and 2AL (from Stoa) were identified by interval analysis using a LOD threshold of 3.0. These two quantitative trait loci (QTL) have been assigned the gene designations QFhs.ndsu-3B and QFhs.ndsu-2A, respectively. Recombinant inbred lines with these two QTL had a median severity of 20.9%, compared with 36.2% for all RI lines. The best RFLP marker in the 3BS region explained 15.4% of the variation and a multiple regression model consisting of three QTL explained 29.5% of the variation. These results indicate that resistance to FHB is inherited in a quantitative manner and that marker-assisted selection may aid the development of FHB-resistant cultivars. Citing Literature Volume39, Issue3May–June 1999Pages 805-811 RelatedInformation