A digital ion trap mass spectrometer coupled with atmospheric pressure ion sources

Abstract In a digital ion trap (DIT), the quadrupole trapping and excitation waveforms are generated by the rapid switching between discrete d.c. voltage levels. As the timing of the switch can be controlled precisely by digital circuitry, the approach provides an opportunity to generate mass spectra by means of a frequency scan in contrast to the conventional voltage scan, thus providing a wider mass range of analysis. An instrument has been constructed which employs a ‘non‐stretched’ ion trap and the field fault around the aperture of the end‐cap electrode can be corrected electronically using a field‐adjusting electrode. The ion trap was coupled with electrospray ionization (ESI) and atmospheric pressure matrix‐assisted laser desorption/ionization (AP‐MALDI) sources to demonstrate the capability of the digital method. AP‐MALDI mass spectra of singly charged ions with mass‐to‐charge ratios upto 17 000 Th were generated using a trapping voltage of only 1000 V. Forward and reverse mass scans at resolutions up to 19 000 and precursor ion isolation at resolutions up to 3500 with subsequent tandem mass spectrometric analysis were demonstrated. The method of generating the digital waveforms and period scan is described. Discussion of the issues of mass range, scan speed, ion trapping efficiency and collision‐induced dissociation efficiency are also provided. Copyright © 2004 John Wiley & Sons, Ltd.