自动调节
旅客8
甲状腺
DNA
细胞生物学
生物
内科学
转录因子
计算生物学
癌症研究
化学
医学
基因
遗传学
血压
作者
Alessandra di Gennaro,Olga Spadaro,Maria Giuseppina Baratta,Mario De Felice,Roberto Di Lauro
出处
期刊:Thyroid
[Mary Ann Liebert, Inc.]
日期:2012-10-19
卷期号:23 (4): 488-496
被引量:14
标识
DOI:10.1089/thy.2012.0357
摘要
Organogenesis of the thyroid gland requires the Pax8 protein. Absence or reduction of Pax8 results in congenital hypothyroidism in animal models and humans, respectively. This study aims at elucidating the regulatory mechanism leading to the expression of Pax8 in thyroid cells.The murine Pax8 gene promoter was functionally dissected by mutagenesis and transfection in the thyroid cell line FRTL-5. Nuclear factors important for thyroid-specific gene expression were identified by DNA-binding assays.We show that Pax8 binds to and controls the expression of its own promoter. Furthermore, we identify a novel, thyroid-specific, DNA-binding activity (denominated nTTF [for novel Thyroid Transcription Factor]) that recognizes a specific region of the Pax8 promoter.The Pax8 promoter appears to be autoregulated, a feature that might be responsible for the haploinsufficiency displayed by this gene.
科研通智能强力驱动
Strongly Powered by AbleSci AI