Novel method for preparation of β-cyclodextrin/grafted chitosan and it's application

A novel technique for preparation of β-cyclodextrin-grafted chitosan was carried out by reacting β-cyclodextrin citrate (β-CD citrate) with chitosan. β-Cyclodextrin citrate was synthesis by esterifying β-cyclodextrin (β-CD) with citric acid (CA) in presence or absence of sodium hypophosphite as a catalyst in a semidry process. Different factors affecting preparation of β-CD citrates were studied to obtain β-CD citrate with high carboxyl content, such factors include reaction temperature, citric acid concentration, material to liquor ratio and duration. β-Cyclodextrin/grafted chitosan was prepared by coupling β-CD citrate with chitosan dissolved in different formic acid solutions having different concentrations. The reacting ingredients were subjected to various reaction conditions to attain the optimum condition. β-Cyclodextrin/grafted chitosan were evaluated by measuring the nitrogen content of both chitosan and grafted chitosan. Chitosan and β-cyclodextrin/grafted chitosan, having different molecular weights, were evaluated as antimicrobial agents for different microorganisms such as, Bacillus Megaterium, Pseudomonas Fragi, Bacillus Cereus Staphylococcus Aureus, Escherichia Ecoli and Aeromonas hydra.