斑马鱼
生物
达尼奥
转基因
绿色荧光蛋白
分子生物学
基因
基因表达
肝再生
转基因小鼠
细胞生物学
生物化学
再生(生物学)
作者
Guor Mour Her,Chia‐Chang Chiang,Wenya Chen,Jen‐Leih Wu
出处
期刊:FEBS Letters
[Wiley]
日期:2003-02-21
卷期号:538 (1-3): 125-133
被引量:204
标识
DOI:10.1016/s0014-5793(03)00157-1
摘要
Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5'-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5'-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42-51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development.
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