肝移植
缺血
医学
移植
肝再生
冷缺血
免疫组织化学
内科学
肝切除术
肿瘤坏死因子α
病理
男科
胃肠病学
免疫学
内分泌学
再灌注损伤
生物
外科
再生(生物学)
切除术
细胞生物学
作者
Quan Qi,Zu-Yao Yang,Kuansheng Ma,Qiang Lü,S.-G. Wang,X.-W. Li,Fu Xia,W. Liu,Ping Bie
出处
期刊:Genetics and Molecular Research
[Genetics and Molecular Research]
日期:2013-01-01
卷期号:12 (3): 4003-4008
被引量:3
标识
DOI:10.4238/2013.september.27.1
摘要
To study the impact of cold ischemia on tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10) expression after liver transplantation, a stable model of partial liver transplantation in rats was established. The experimental animals were divided into the following groups: a partial hepatectomy control group, a group that received partial liver transplantation after 30 min of cold ischemia (experimental group A), and a group that received a partial liver transplantation after 10 h of cold ischemia (experimental group B). The survival rate was observed in each group. The liver tissue was sampled 1, 2, and 4 days after surgery, and immunohistochemical detection of proliferating cell nuclear antigen TNF-α and IL-10 was performed. The correlation between liver regeneration and TNF-α and IL-10 expression was analyzed, and the impact of the 2 cytokines on rat liver regeneration after liver transplantation was evaluated. The survival rates of rats in the partial hepatectomy control group, in the group that received a partial liver transplantation after 30 min of cold ischemia, and the group that received a partial liver transplantation after 10 h of cold ischemia were 100, 70, and 33.3%, respectively. The expression of proliferating cell nuclear antigen and TNF-α was decreased (P < 0.05), and IL-10 expression was increased (P < 0.05) in animals that received a partial liver transplant after 10 h of cold ischemia compared with that in the animals that received a partial liver transplant after 30 min of cold ischemia. We conclude that with the extension of cold ischemic time, liver regeneration and survival rate after liver transplantation decreased. TNF-α and IL-10 play important regulatory roles in the regeneration process of transplanted livers.
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