Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

The Chemical RecordVolume 16, Issue 3 p. 1436-1455 Personal Account Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities Nazzoly Rueda, Nazzoly Rueda Departamento de Biocatálisis, ICP-CSIC C/Marie Curie 2, Campus UAM-CSIC, Cantoblanco, 28049 Madrid, Spain Escuela de Química, Grupo de investigación en Bioquímica y Microbiología (GIBIM) Edificio Camilo Torres 210, Universidad Industrial de Santander, CEP, 680001 Bucaramanga, ColombiaSearch for more papers by this authorJose C. S. dos Santos, Jose C. S. dos Santos Departamento de Biocatálisis, ICP-CSIC C/Marie Curie 2, Campus UAM-CSIC, Cantoblanco, 28049 Madrid, Spain Instituto de Engenharias e Desenvolvimento Sustentável Universidade da Integração Internacional da Lusofonia Afro-Brasileira, CEP, 62785-000 Acarape, CE, BrazilSearch for more papers by this authorClaudia Ortiz, Claudia Ortiz Escuela de Microbiología, Universidad Industrial de Santander, Bucaramanga, ColombiaSearch for more papers by this authorRodrigo Torres, Rodrigo Torres Escuela de Química, Grupo de investigación en Bioquímica y Microbiología (GIBIM) Edificio Camilo Torres 210, Universidad Industrial de Santander, CEP, 680001 Bucaramanga, ColombiaSearch for more papers by this authorOveimar Barbosa, Oveimar Barbosa Departamento de Química, Facultad de Ciencias Universidad del Tolima, Ibagué, ColombiaSearch for more papers by this authorRafael C. Rodrigues, Rafael C. Rodrigues Biocatalysis and Enzyme Technology Laboratory, Institute of Food Science and Technology Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500 P.O. Box 15090, Porto Alegre, RS, BrazilSearch for more papers by this authorÁngel Berenguer-Murcia, Ángel Berenguer-Murcia Instituto Universitario de Materiales Departamento de Química Inorgánica Universidad de Alicante Campus de San Vicente del Raspeig, Ap. 99 - 03080 Alicante, SpainSearch for more papers by this authorRoberto Fernandez-Lafuente, Corresponding Author Roberto Fernandez-Lafuente Departamento de Biocatálisis, ICP-CSIC C/Marie Curie 2, Campus UAM-CSIC, Cantoblanco, 28049 Madrid, SpainE-mail: rfl@icp.csic.esSearch for more papers by this author Nazzoly Rueda, Nazzoly Rueda Departamento de Biocatálisis, ICP-CSIC C/Marie Curie 2, Campus UAM-CSIC, Cantoblanco, 28049 Madrid, Spain Escuela de Química, Grupo de investigación en Bioquímica y Microbiología (GIBIM) Edificio Camilo Torres 210, Universidad Industrial de Santander, CEP, 680001 Bucaramanga, ColombiaSearch for more papers by this authorJose C. S. dos Santos, Jose C. S. dos Santos Departamento de Biocatálisis, ICP-CSIC C/Marie Curie 2, Campus UAM-CSIC, Cantoblanco, 28049 Madrid, Spain Instituto de Engenharias e Desenvolvimento Sustentável Universidade da Integração Internacional da Lusofonia Afro-Brasileira, CEP, 62785-000 Acarape, CE, BrazilSearch for more papers by this authorClaudia Ortiz, Claudia Ortiz Escuela de Microbiología, Universidad Industrial de Santander, Bucaramanga, ColombiaSearch for more papers by this authorRodrigo Torres, Rodrigo Torres Escuela de Química, Grupo de investigación en Bioquímica y Microbiología (GIBIM) Edificio Camilo Torres 210, Universidad Industrial de Santander, CEP, 680001 Bucaramanga, ColombiaSearch for more papers by this authorOveimar Barbosa, Oveimar Barbosa Departamento de Química, Facultad de Ciencias Universidad del Tolima, Ibagué, ColombiaSearch for more papers by this authorRafael C. Rodrigues, Rafael C. Rodrigues Biocatalysis and Enzyme Technology Laboratory, Institute of Food Science and Technology Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500 P.O. Box 15090, Porto Alegre, RS, BrazilSearch for more papers by this authorÁngel Berenguer-Murcia, Ángel Berenguer-Murcia Instituto Universitario de Materiales Departamento de Química Inorgánica Universidad de Alicante Campus de San Vicente del Raspeig, Ap. 99 - 03080 Alicante, SpainSearch for more papers by this authorRoberto Fernandez-Lafuente, Corresponding Author Roberto Fernandez-Lafuente Departamento de Biocatálisis, ICP-CSIC C/Marie Curie 2, Campus UAM-CSIC, Cantoblanco, 28049 Madrid, SpainE-mail: rfl@icp.csic.esSearch for more papers by this author First published: 11 May 2016 https://doi.org/10.1002/tcr.201600007Citations: 146Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Chemical modification of enzymes and immobilization used to be considered as separate ways to improve enzyme properties. This review shows how the coupled use of both tools may greatly improve the final biocatalyst performance. Chemical modification of a previously immobilized enzyme is far simpler and easier to control than the modification of the free enzyme. Moreover, if protein modification is performed to improve its immobilization (enriching the enzyme in reactive groups), the final features of the immobilized enzyme may be greatly improved. Chemical modification may be directed to improve enzyme stability, but also to improve selectivity, specificity, activity, and even cell penetrability. Coupling of immobilization and chemical modification with site-directed mutagenesis is a powerful instrument to obtain fully controlled modification. Some new ideas such as photoreceptive enzyme modifiers that change their physical properties under UV exposition are discussed. Citing Literature Volume16, Issue3June 2016Pages 1436-1455 RelatedInformation