[Development of polycomponent metabolite probiotic].

干酪乳杆菌 益生菌 瑞士乳杆菌 微生物学 乳酸菌 粪肠球菌 生物 抗菌剂 金黄色葡萄球菌 化学 细菌 食品科学 发酵 遗传学
作者
Kulakova IuV,А. В. Алешкин,Afanas'ev Ss,Zhilenkova Og
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期刊:PubMed 卷期号: (5): 80-6
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Construct composition of polycomponent probiotic based on lactobacilli cultural fluid (CF) supernatant.Species membership of lactobacilli strains was specified by analysis of nucleotide sequences of 16S RNA genes. For comparison of probiotic effect of CF, supernatant and ultrafiltrate of a consortium of lactobacilli strains, studies of specter of peptides, amino- and other organic acids contained in them as well as profile of bactericidal and fungicidal activity was carried out. RESULTS; Technology of production of amicrobial probiotic agent based on CF of the consortium of lactobacilli strains was developed. During determination of concentration of metabolites of protein nature analogy of parameters was shown in CF supernatant and native lactobacilli cultures. In both the studied supernatants and CF the same set of organic acids was also reported to be present. During comparison of antimicrobial and anticandidosis activity of lactobacilli native culture and CF supernatant against Staphylococcus aureus 209, Enterococcus faecalis 1154 and Candida albicans 5 test-strains, an equivalent effect of both samples was shown.Polycomponent metabolite probiotic based on CF supernatant of Lactobacillus helveticus NKJC, Lactobacillus helveticus JCH and Lactobacillus casei KAA strains completely retains bactericidal and fungicidal activity of the native culture of the consortium of strains and at the same time lacks disadvantages of compositions containing live microorganisms.

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