Technology of Homogenate Extraction of Camptothecin and 10-Hydroxycamptothecin in Camptotheca acuminata Seed

The method of homogenate extraction of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata seed was studied,and comparisons of this method with other extractive methods such as sonication,reflux,soakage,shaking in water bath were conducted.For the homogenate extraction,the optimum technology parameters were found as following:homogenate for 8 min with 55% ethanol at a ratio of material to liquid of 1∶15(g∶mL).By using the optimized parameters,the extraction yield of camptothecin and 10-hydroxycamptothecin was 0.801‰ and 0.546‰,respectively.Moreover,homogenated extraction required less extraction time and had high yield,which was an efficient method for extracting camptothecin and 10-hydroxycamptothecin.