Measuring Cell–Cell Binding Using Flow-Cytometry

AbstractCell–cell adhesion mediates a number of competitive and cooperative microbial interactions. Fluorescence labeling and flow cytometry techniques allow us to observe and measure these interactions rapidly and easily. Here, we describe a method to quantify cell–cell adhesion events between two differentially labeled cell populations.Key wordsCdiAcell–cell bindingContact-dependent growth inhibitionFlow cytometryFluorescent proteinsSelf/nonself recognition