细胞毒性T细胞
细胞毒性
化学
生物化学
蛇毒
细胞凋亡
Jurkat细胞
毒液
生物
免疫学
体外
T细胞
免疫系统
作者
Patrícia Helena Ribeiro,Juliana P. Zuliani,Carla Freire Celêdonio Fernandes,Leonardo A. Calderón,Rodrigo G. Stábeli,Auro Nomizo,Andreimar M. Soares
标识
DOI:10.1016/j.ijbiomac.2016.07.022
摘要
BaltLAAO-I, an L-amino acid oxidase isolated from Bothrops alternatus, is a glycoprotein enzyme with a pI-5.3, 15% sugar and a related molecular mass of 66,000Da in its monomeric form, and 123,000Da in its dimeric form. The objective of this study is to describe the cytotoxicity activity induced by BaltLAAO-I isolated from Bothrops alternatus venom and its possible mechanism of action on tumor cells. Our results clearly depict that BaltLAAO-I has a strong selective cytotoxic activity on tumor cell lines (JURKAT, SK-BR-3 and B16F10). On the other hand, the results show low cytotoxicity on human peripheral blood mononuclear cells. Furthermore, our findings demonstrate that BaltLAAO-I induces the apoptosis of tumor cell lines through a cytotoxic activity exerted by a generation of reactive oxygen intermediates. All in all, the data indicate that LAAOs exert a selective cytotoxic role on tumor cells, demonstrating a great potential for future use in clinical therapy.
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