To develop a method for elucidating " chromatography-efficacy" relation of the extract of Zanthoxylum nitidum on the gastric cancer cells.After obtaining the tumor inhibition rate and fingerprint peak data through MTT and HPLC, "chromatography-efficacy" relation was established by an appropriate statistical method.The gastric cancer "chromatography-efficacy" relation of Z. nitidum was established by step-back technique.The "chromatography-efficacy" relation has statistically significant and practical significance, so it has reference value in some way.