纳米颗粒
纳米探针
胶体金
生物传感器
原位
分子信标
分析物
荧光
拉曼光谱
银纳米粒子
拉曼散射
电化学发光
表面等离子共振
作者
Yuanyuan Yao,Hongding Zhang,Tongtong Tian,Yixin Liu,Rendan Zhu,Ji Ji,Baohong Liu
出处
期刊:Talanta
[Elsevier]
日期:2021-12-01
卷期号:235: 122728-
被引量:3
标识
DOI:10.1016/j.talanta.2021.122728
摘要
Abstract With the emergence of microRNA (miRNA) as a key player in early clinical disease diagnosis, development of rapidly sensitive and quantitative miRNA detection methods are imperative. Herein, a label-free SERS assay coupled with duplex-specific nuclease (DSN) signal amplification strategy was proposed for facilely ultrasensitive and quantitative analysis of miRNA-21. Firstly, magnetic beads assembled with excessive capture DNA were utilized to hybridize the target miRNA-21. These DNA-RNA heteroduplexes were cleaved by DSN to generate small nucleotide fragments into the supernatant and the miRNA-21 released and rehybridized another DNA, going to the next DSN cycle. Consequently, numerous of small nucleotide fragments of capture DNA were released from magnetic beads and the miRNA-21 signal was transferred and amplified by the SERS signals of total phosphate backbones which are abundant in nucleotide. Furthermore, iodide-modified Ag nanoparticles (AgINPs) was employed to generate a strong and reproducible SERS signal. The proposed method displayed excellent performance for miRNA-21 detection with the linear range from 0.33 fM to 3.3 pM, and a lower detection limit of 42 aM. Moreover, this strategy exhibited effectively base discrimination capability and was successfully applied for monitoring the expression levels of miRNA-21 in different cancer cell lines and human serum.
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