Hyperproduction of urate oxidase (BEM-2) by optimizing liquid state fermentation medium: Hyperproduction of urate oxidase (BEM-2)

Abstract Urate oxidase is a crucial enzyme for uric acid estimation in different biological fluid. The main purpose of this research washyperproduction of industrially important urate oxidase by liquid state fermentation (LSF) after mutagenesis of Bacillus subtilis by using different chemical mutagens. At 180 minutes dose rate of ethyl methane sulfonate treated mutagen strain (BEM-2) was exhibited good results for maximum production of urate oxidase. The selected strain BEM-2 was used for optimum production of enzyme.  The fermentation medium of wild and mutated strains was individually optimized. The results exhibited that LSF with substrate concentration (0.5 %), pH8.5 at 35 oCtemperature was produced urate oxidase after 36 hours by wild (40.16±0.91 U/mg) and mutant strains (12.72±1.26 U/mg).the study revealed that 0.3% yeast extract was the best nitrogen source for both wild and mutant derived urate oxidase.When 0.2%peptone and 0.5%sodium nitrate was added in the medium. The effect of various carbon sources were compared and revealed that 2%sucrose as carbon source was the best. It was produced optimum yield of enzyme by wild and mutant derived strains. it was concluded that the nutritional requirements of both wild and mutated strain. were same.