火球菌属
阿尔戈瑙特
核酸
化学
DNA
劈开
核糖核酸
核苷酸
分子信标
突变体
劈理(地质)
分子生物学
生物化学
计算生物学
生物
古细菌
基因
寡核苷酸
古生物学
断裂(地质)
小干扰RNA
作者
Ruyi He,Longyu Wang,Fei Wang,Wenqiang Li,Yang Liu,Aitao Li,Yuan Wang,Wuxiang Mao,Chao Zhai,Lixin Ma
摘要
We demonstrate that short single-stranded DNA generated by Pyrococcus furiosus Argonaute (PfAgo) can initiate a second round of cleavage. Based on this principle, we established a molecular diagnostic method, termed PfAgo-mediated Nucleic acid Detection (PAND). This method could detect DNA at attomolar sensitivities, distinguish single-nucleotide mutants and accomplish multiplexed detection.
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