同轴
湍流
体外
材料科学
制作
阿托伐他汀
生物物理学
生物医学工程
纳米技术
医学
生物
病理
内科学
机械
计算机科学
生物化学
物理
电信
替代医学
作者
Ge Gao,Wonbin Park,Byoung Soo Kim,Minjun Ahn,Suhun Chae,Dong‐Woo Cho,Jisoo Kim,Jae Yeon Lee,Jinah Jang,Dong‐Woo Cho
标识
DOI:10.1002/adfm.202008878
摘要
Abstract As the main precursor of cardiovascular diseases, atherosclerosis is a complex inflammatory disorder that preferentially occurs in stenotic, curved, and branched arterial regions. Although various in vitro models are established to understand its pathology, reconstructing the native atherosclerotic environment that involves both co‐cultured cells and local turbulent flow singling remains challenging. This study develops an arterial construct via in‐bath coaxial cell printing that not only facilitates the direct fabrication of three‐layered conduits with tunable geometry and dimensions but also maintains structural stability. Functional vascular tissues, which respond to various stimulations that induce endothelial dysfunction, are rapidly generated in the constructed models. The presence of multiple vascular tissues under stenotic and tortuous turbulent flows allows the recapitulation of hallmark events in early atherosclerosis under physiological conditions. Furthermore, the fabricated models are utilized to investigate the individual and synergistic functions of cell co‐culture and local turbulent flows in regulating atherosclerotic initiation, as well as the dose‐dependent therapeutic effect of atorvastatin. These outcomes suggest that the constructed atherosclerotic model via a novel fabrication strategy is a promising platform to elucidate the pathophysiology of atherosclerosis and seek effective drugs and therapies.
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