基因簇
稻黄单胞菌
生物
基因
遗传学
黄单胞菌
基因组
R基因
植物抗病性
生物化学
苯丙素
生物合成
作者
Shuangqian Shen,Peng Meng,Hong Fang,Zixuan Wang,Shen Zhou,Xinyu Jing,Meng Yuan,Chenkun Yang,Hao Guo,Yufei Li,Long Lei,Yuheng Shi,Yangyang Sun,Xianqing Liu,Congping Xu,Takayuki Tohge,Meng Yuan,Alisdair R. Fernie,Yuese Ning,Guo‐Liang Wang,Jie Luo
标识
DOI:10.1016/j.scib.2021.03.015
摘要
Genomic clustering of non-homologous genes for the biosynthesis of plant defensive compounds is an emerging theme, but insights into their formation and physiological function remain limited. Here we report the identification of a newly discovered hydroxycinnamoyl tyramine (HT) gene cluster in rice. This cluster contains a pyridoxamine 5'-phosphate oxidase (OsPDX3) producing the cofactor pyridoxal 5'-phosphate (PLP), a PLP-dependent tyrosine decarboxylase (OsTyDC1), and two duplicated hydroxycinnamoyl transferases (OsTHT1 and OsTHT2). These members were combined to represent an enzymological innovation gene cluster. Natural variation analysis showed that the abundance of the toxic tyramine intermediate of the gene cluster among different rice accessions is mainly determined by the coordinated transcription of OsTyDC1 and OsTHT1. Further pathogen incubation assays demonstrated that the end products of the HT gene cluster displayed enhanced resistance to the bacterial pathogen Xanthomonas oryzae pv. Oryzae (Xoo) and fungal pathogen Magnaporthe oryzae (M. oryzae), and the enhanced resistance is associated with the boost of phytoalexins and the activation of defense response. The unique presence of the HT gene cluster in Oryza AA genome, together with the enrichment of transposon elements within this gene cluster region, provides an evolutionary background to accelerate cluster member combinations. Our study not only discovered a gene cluster involved in the phenylpropanoid metabolism but also addressed the key aspects of gene cluster formation. In addition, our results provide a new metabolic pool for plant defense against pathogens.
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