[Clinical significance of exosomal miR-1231 in pancreatic cancer].

胰腺癌 医学 临床意义 微泡 阶段(地层学) 外体 癌症 内科学 小RNA 肿瘤科 癌症研究 转移 生物 基因 生物化学 古生物学
作者
Shilin Chen,Min Ma,Lei Yan,Shuhan Xiong,Zhuo Liu,Sha Li,Teng Liu,Song Shang,Yu Zhang,Hui Zeng,Hailong Xie,Chaohui Zuo
出处
期刊:PubMed 卷期号:41 (1): 46-49 被引量:25
标识
DOI:10.3760/cma.j.issn.0253-3766.2019.01.008
摘要

Objective: To investigate the expression and clinical significance of exosomal miR-1231 in plasma of pancreatic cancer (PC) patients and pancreatic cancer cells. Methods: A total of 16 patients who were diagnosed with pancreatic cancer in Hunan Cancer Hospital were collected from April 2016 to August 2017. Meanwhile, 16 healthy volunteers were recruited as the healthy control group at the same period. The plasma exosomes were extracted, and the levels of miR-1231 were detected by qRT-PCR in PC and healthy control groups. Moreover, the clinicopathological significance of exosomal miR-1231 expression was analyzed. Furthermore, the expression of exosomal miR-1231 was detected in several pancreatic cancer cells (MIA PaCa-2, PANC-1, SW1990, AsPC-1 and BxPc-3) and two normal pancreatic epithelial cells (HPDE and human primary pancreatic epithelial cell). Results: qRT-PCR results showed that the expression level of miR-1231 in plasma exosomes of pancreatic cancer patients (1.06±0.46) was significantly lower than that in healthy controls (2.30±0.99; P<0.05). The levels of exosomal miR-1231 in patients with stage Ⅰ-Ⅱ (1.515±0.531), no distant metastasis (1.236±0.461) and no lymph node metastasis (1.337±0.522) were significantly higher than those with stage Ⅲ-Ⅳ (0.848±0.224), distant metastasis (0.757±0.278) and lymph node metastasis (0.838±0.261), respectively (P<0.05 for all). In addition, there were no correlation between exosomal miR-1231 expression and age, sex, smoking history, CA19-9 levels and tumor sites (P>0.05). Furthermore, the expression level of exosomal miR-1231 in pancreatic cancer cell lines (0.142±0.135) was significantly lower than that in normal epithelial cells (1.127±0.179; P<0.05). Conclusions: The downregulation of exosomal miR-1231 in plasma of pancreatic cancer patients and pancreatic cancer cells suggests that it is related to the initiation and development of PC. It may be a new diagnostic and prognostic marker for PC.目的: 探讨miR-1231在胰腺癌患者血浆和胰腺癌细胞外泌体中的表达及其临床意义。 方法: 选取2016年4月至2017年8月在湖南省肿瘤医院确诊为胰腺癌的患者16例,选取16例同期健康志愿者作为正常对照组。收集16例胰腺癌患者和16例健康志愿者的血浆,采用实时荧光定量PCR法检测血浆外泌体中miR-1231的表达,分析外泌体中miR-1231的表达水平与胰腺癌患者临床病理特征的关系。分析胰腺癌细胞系MIA PaCa-2、PANC-1、SW1990、AsPC-1和BxPc-3以及正常胰腺上皮细胞(人胰腺导管上皮细胞和原代人正常胰腺上皮细胞)外泌体中miR-1231的表达。 结果: 实时荧光定量PCR检测结果显示,胰腺癌患者血浆外泌体中miR-1231的表达水平为1.06±0.46,低于健康志愿者(2.30±0.99),差异有统计学意义(P<0.05)。miR-1231在Ⅰ~Ⅱ期和Ⅲ~Ⅳ期胰腺癌患者血浆外泌体中的表达水平分别为1.515±0.531和0.848±0.224,miR-1231在有、无远处转移的胰腺癌患者血浆外泌体中的表达水平分别为0.757±0.278和1.236±0.461,miR-1231在有、无淋巴结转移的胰腺癌患者血浆外泌体中的表达水平为0.838±0.261和1.337±0.522,差异均有统计学意义(均P<0.05)。胰腺癌患者血浆外泌体中miR-1231在不同年龄、性别、有无吸烟史、糖链抗原19-9(CA19-9)浓度和肿瘤部位的胰腺癌患者血浆外泌体中的表达水平差异均无统计学意义(均P>0.05),而在不同淋巴转移情况、不同远处转移情况和不同临床分期的胰腺癌患者血浆外泌体中的表达差异均有统计学意义(均P<0.05)。miR-1231在胰腺癌细胞外泌体中的表达水平为0.142±0.135,低于正常胰腺上皮细胞(1.127±0.179),差异有统计学意义(P<0.05)。 结论: 胰腺癌患者血浆和胰腺癌细胞外泌体中miR-1231的低表达可能与胰腺癌的发生发展有关,外泌体中miR-1231的表达可能成为胰腺癌新的诊断标志物或预后因子。.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Tmac完成签到,获得积分10
刚刚
粗暴的遥完成签到,获得积分10
刚刚
万能图书馆应助xiaomi采纳,获得10
1秒前
AGuang应助奇奇淼采纳,获得10
1秒前
2秒前
淡淡晓夏发布了新的文献求助10
2秒前
2秒前
魔王完成签到,获得积分10
2秒前
3秒前
3秒前
阳光万声完成签到,获得积分10
4秒前
fusheng发布了新的文献求助10
4秒前
阳光项链完成签到,获得积分10
4秒前
奶冻完成签到,获得积分10
4秒前
Tmac发布了新的文献求助10
5秒前
邓佳鑫Alan应助lvshuye采纳,获得10
5秒前
5秒前
6秒前
wayne完成签到,获得积分10
6秒前
6秒前
7秒前
情怀应助abcdefg采纳,获得10
7秒前
星辰发布了新的文献求助50
7秒前
酷波er应助开心超人采纳,获得10
7秒前
Kristina完成签到,获得积分10
8秒前
8秒前
9秒前
雪落发布了新的文献求助10
9秒前
10秒前
晨曦完成签到,获得积分10
10秒前
10秒前
张爱学发布了新的文献求助10
10秒前
10秒前
yuyu发布了新的文献求助10
11秒前
AJIN发布了新的文献求助10
11秒前
12秒前
12秒前
善学以致用应助十八冠六采纳,获得10
13秒前
13秒前
13秒前
高分求助中
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Cognitive Neuroscience: The Biology of the Mind (Sixth Edition) 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 588
Christian Women in Chinese Society: The Anglican Story 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3961589
求助须知:如何正确求助?哪些是违规求助? 3507917
关于积分的说明 11138698
捐赠科研通 3240341
什么是DOI,文献DOI怎么找? 1790929
邀请新用户注册赠送积分活动 872649
科研通“疑难数据库(出版商)”最低求助积分说明 803306