Molecular detection and antimicrobial activity of Endophytic fungi isolated from a medical plant Rosmarinus officinalis

Endophytes are tiny organisms present in living tissues of distinct plants\nand have been extensively studied for their endophytic microbial complement.\nRoots of Rosmarinus officinalis were subjected to the isolation of endophytic\nfungi and screened for antimicrobial activity against Gram-positive\n(Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia\ncoli, Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria. Genomic DNA from\nactive fungal strain of Trichoderma harzianum was isolated, and the internal\ntranscribed spacer (ITS) region was amplified using ITS4 and ITS5 primers and\nsequenced for genetic inference in fungus. The crude extract of T. harzianum\nisolate with Ethyl acetate was showed significant antimicrobial activity\nagainst P. aeruginosa, S. aureus, K. pneumonia, B. subtilis and E. coli. The\nantimicrobial activity was highest against P. aeruginosa at concentration of 40\nmicrogram/ ml, followed by S. aureus and K. pneumonia at the same\nconcentration. The lowest antimicrobial activity was against by S. aureus at\nconcentration of 60 microgram/ ml. The current study is confirmed that the\nantimicrobial activity is due to bioactive compounds founded in endophytic\nfungi.\n