Reduction of mitochondrial 3-oxoacyl-ACP synthase (OXSM) by hyperglycemia is associated with deficiency of α-lipoic acid synthetic pathway in kidney of diabetic mice

内分泌学 发病机制 内科学 糖尿病 硫辛酸 2型糖尿病 医学 生物 化学 生物化学 抗氧化剂
作者
Ting Gao,Shengnan Qian,Shuang Shen,Xiaoying Zhang,Junli Liu,Weiping Jia,Zhong Chen,Jianping Ye
出处
期刊:Biochemical and Biophysical Research Communications [Elsevier]
卷期号:512 (1): 106-111 被引量:16
标识
DOI:10.1016/j.bbrc.2019.02.155
摘要

LA (alpha-Lipoic acid) deficiency represents a risk factor in the pathogenesis of diabetic complications as synthetic LA is routinely used in the treatment of the complications in patients. The mechanism underlying LA deficiency remains elusive in the diabetic conditions. In the present study, we investigated the synthetic pathway of LA in both type 1 and 2 diabetic mice. LA deficiency was observed with a reduction in lipoylation of pyruvate dehydrogenase in the kidney of streptozocin-induced diabetic mice. Proteins of three enzymes (MCAT, OXSM and LIAS) in the LA synthetic pathway were examined in the kidney. A reduction was observed in OXSM, but not in the other two. In a 24h study in the cell culture, mRNA and protein of OXSM were transiently reduced by a high concentration of glucose (35 mM), and persistently decreased by TNF-α (20 nM). The high glucose effect was observed with the OXSM reduction in the kidney of db/db mice (type 2 diabetes model). The TNF-α effect was observed with OXSM reduction in the fat tissue of diet-induced obese mice. The result suggest that inhibition of OXSM by hyperglycemia and inflammation may contribute to the LA deficiency in the diabetic complications. The OXSM reduction suggests a new mechanism for the mitochondrial dysfunction in the pathogenesis of diabetic complications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
夜白完成签到 ,获得积分10
1秒前
kb发布了新的文献求助10
1秒前
啦啦啦发布了新的文献求助30
3秒前
托丽莲睡拿完成签到,获得积分10
3秒前
3秒前
4秒前
陈M雯发布了新的文献求助10
5秒前
6秒前
苏州小北完成签到,获得积分20
7秒前
8秒前
顾矜应助phy采纳,获得10
8秒前
8秒前
qyz发布了新的文献求助10
9秒前
苏州小北发布了新的文献求助10
9秒前
9秒前
nmamtf完成签到,获得积分10
10秒前
10秒前
11秒前
背后的铭发布了新的文献求助10
11秒前
刘才华发布了新的文献求助10
13秒前
轩辕白竹发布了新的文献求助10
14秒前
laaa发布了新的文献求助10
15秒前
布丁仔发布了新的文献求助10
15秒前
Amorfati完成签到,获得积分20
16秒前
孙意冉发布了新的文献求助10
16秒前
自由半雪应助qyz采纳,获得10
16秒前
xh发布了新的文献求助10
16秒前
18秒前
19秒前
20秒前
我爱金哥关注了科研通微信公众号
21秒前
科目三应助无限猕猴桃采纳,获得10
22秒前
完美世界应助aosik采纳,获得10
23秒前
Su完成签到,获得积分10
23秒前
24秒前
小马甲应助晴空之下采纳,获得10
24秒前
24秒前
Lucas应助laaa采纳,获得10
25秒前
清脆的土豆应助nicezhutou采纳,获得30
25秒前
25秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Write Like a Chemist: A Guide and Resource (第二版) 600
Mixed-anion Compounds 600
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
Earth System Geophysics 500
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Geochemistry, 2nd Edition 地球化学经典教科书第二版 401
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3199994
求助须知:如何正确求助?哪些是违规求助? 2849782
关于积分的说明 8069853
捐赠科研通 2513573
什么是DOI,文献DOI怎么找? 1346379
科研通“疑难数据库(出版商)”最低求助积分说明 640227
邀请新用户注册赠送积分活动 610102