Reduction of mitochondrial 3-oxoacyl-ACP synthase (OXSM) by hyperglycemia is associated with deficiency of α-lipoic acid synthetic pathway in kidney of diabetic mice

内分泌学 发病机制 内科学 糖尿病 硫辛酸 2型糖尿病 医学 生物 化学 生物化学 抗氧化剂
作者
Ting Gao,Shengnan Qian,Shuang Shen,Xiaoying Zhang,Junli Liu,Weiping Jia,Zhong Chen,Jianping Ye
出处
期刊:Biochemical and Biophysical Research Communications [Elsevier]
卷期号:512 (1): 106-111 被引量:16
标识
DOI:10.1016/j.bbrc.2019.02.155
摘要

LA (alpha-Lipoic acid) deficiency represents a risk factor in the pathogenesis of diabetic complications as synthetic LA is routinely used in the treatment of the complications in patients. The mechanism underlying LA deficiency remains elusive in the diabetic conditions. In the present study, we investigated the synthetic pathway of LA in both type 1 and 2 diabetic mice. LA deficiency was observed with a reduction in lipoylation of pyruvate dehydrogenase in the kidney of streptozocin-induced diabetic mice. Proteins of three enzymes (MCAT, OXSM and LIAS) in the LA synthetic pathway were examined in the kidney. A reduction was observed in OXSM, but not in the other two. In a 24h study in the cell culture, mRNA and protein of OXSM were transiently reduced by a high concentration of glucose (35 mM), and persistently decreased by TNF-α (20 nM). The high glucose effect was observed with the OXSM reduction in the kidney of db/db mice (type 2 diabetes model). The TNF-α effect was observed with OXSM reduction in the fat tissue of diet-induced obese mice. The result suggest that inhibition of OXSM by hyperglycemia and inflammation may contribute to the LA deficiency in the diabetic complications. The OXSM reduction suggests a new mechanism for the mitochondrial dysfunction in the pathogenesis of diabetic complications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
kingchen81完成签到,获得积分10
1秒前
yry完成签到,获得积分10
1秒前
火星上雨珍完成签到,获得积分10
5秒前
zho发布了新的文献求助10
7秒前
852应助刘耿耿采纳,获得10
15秒前
Ava应助学习使我快乐1917采纳,获得10
16秒前
nora完成签到,获得积分10
18秒前
18秒前
小赞芽完成签到,获得积分10
18秒前
如果完成签到 ,获得积分10
19秒前
曾欢完成签到,获得积分10
20秒前
小蘑菇应助祥子的骆驼采纳,获得10
23秒前
23秒前
pzh完成签到 ,获得积分10
24秒前
大模型应助科研通管家采纳,获得10
25秒前
Hello应助科研通管家采纳,获得10
25秒前
Lucas应助科研通管家采纳,获得10
25秒前
坤坤发布了新的文献求助10
28秒前
文静千凡完成签到,获得积分10
28秒前
小不溜完成签到 ,获得积分10
30秒前
科研通AI2S应助asww采纳,获得10
30秒前
zho发布了新的文献求助10
30秒前
四下看发布了新的文献求助10
31秒前
had完成签到,获得积分10
32秒前
32秒前
wangbw完成签到,获得积分10
35秒前
呵呵呵呵呵呵123完成签到,获得积分10
38秒前
38秒前
kid1912完成签到,获得积分10
39秒前
39秒前
沙漠水发布了新的文献求助10
40秒前
LIUYI发布了新的文献求助10
40秒前
科研通AI2S应助Natsume采纳,获得10
42秒前
静心完成签到,获得积分10
42秒前
43秒前
完美世界应助稳重的麦片采纳,获得10
44秒前
are完成签到,获得积分10
44秒前
四下看完成签到,获得积分10
47秒前
47秒前
超帅方盒完成签到,获得积分10
48秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
安全防范技术与工程 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3190778
求助须知:如何正确求助?哪些是违规求助? 2840001
关于积分的说明 8026566
捐赠科研通 2503119
什么是DOI,文献DOI怎么找? 1336731
科研通“疑难数据库(出版商)”最低求助积分说明 637950
邀请新用户注册赠送积分活动 606279