Reduction of mitochondrial 3-oxoacyl-ACP synthase (OXSM) by hyperglycemia is associated with deficiency of α-lipoic acid synthetic pathway in kidney of diabetic mice

内分泌学 发病机制 内科学 糖尿病 硫辛酸 2型糖尿病 医学 生物 化学 生物化学 抗氧化剂
作者
Ting Gao,Shengnan Qian,Shuang Shen,Xiaoying Zhang,Junli Liu,Weiping Jia,Zhong Chen,Jianping Ye
出处
期刊:Biochemical and Biophysical Research Communications [Elsevier]
卷期号:512 (1): 106-111 被引量:16
标识
DOI:10.1016/j.bbrc.2019.02.155
摘要

LA (alpha-Lipoic acid) deficiency represents a risk factor in the pathogenesis of diabetic complications as synthetic LA is routinely used in the treatment of the complications in patients. The mechanism underlying LA deficiency remains elusive in the diabetic conditions. In the present study, we investigated the synthetic pathway of LA in both type 1 and 2 diabetic mice. LA deficiency was observed with a reduction in lipoylation of pyruvate dehydrogenase in the kidney of streptozocin-induced diabetic mice. Proteins of three enzymes (MCAT, OXSM and LIAS) in the LA synthetic pathway were examined in the kidney. A reduction was observed in OXSM, but not in the other two. In a 24h study in the cell culture, mRNA and protein of OXSM were transiently reduced by a high concentration of glucose (35 mM), and persistently decreased by TNF-α (20 nM). The high glucose effect was observed with the OXSM reduction in the kidney of db/db mice (type 2 diabetes model). The TNF-α effect was observed with OXSM reduction in the fat tissue of diet-induced obese mice. The result suggest that inhibition of OXSM by hyperglycemia and inflammation may contribute to the LA deficiency in the diabetic complications. The OXSM reduction suggests a new mechanism for the mitochondrial dysfunction in the pathogenesis of diabetic complications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
田様应助科研通管家采纳,获得10
刚刚
科研通AI2S应助ating采纳,获得10
2秒前
4秒前
秀兰发布了新的文献求助10
4秒前
明亮若枫发布了新的文献求助10
4秒前
WXT1111完成签到,获得积分10
5秒前
劲秉应助keyan采纳,获得10
5秒前
隐形曼青应助动听的囧采纳,获得10
6秒前
7秒前
7秒前
领导范儿应助小郭采纳,获得30
8秒前
活泼的冬寒完成签到,获得积分10
9秒前
毛豆爸爸应助刻苦绿柏采纳,获得10
10秒前
遇桥发布了新的文献求助10
11秒前
stuffmatter应助CUREME采纳,获得10
13秒前
我不是超人完成签到,获得积分10
13秒前
luermei发布了新的文献求助10
13秒前
15秒前
jojo发布了新的文献求助10
19秒前
有魅力听枫完成签到,获得积分10
19秒前
鲨鱼发布了新的文献求助10
19秒前
ZZZ发布了新的文献求助10
21秒前
沐沐完成签到,获得积分10
21秒前
24秒前
24秒前
25秒前
25秒前
共享精神应助浮云采纳,获得10
25秒前
鲨鱼完成签到,获得积分10
25秒前
苏云墨完成签到 ,获得积分10
26秒前
无呜呜发布了新的文献求助30
27秒前
自信秋灵完成签到,获得积分10
29秒前
艺术家发布了新的文献求助10
29秒前
Kvolu29发布了新的文献求助10
29秒前
草莓熊发布了新的文献求助10
30秒前
32秒前
32秒前
科研通AI2S应助酷炫以寒采纳,获得10
34秒前
科目三应助Vincent1990采纳,获得10
34秒前
赘婿应助Lee采纳,获得10
35秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
Handbook on People's China (1957) 400
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3189542
求助须知:如何正确求助?哪些是违规求助? 2838884
关于积分的说明 8022073
捐赠科研通 2501733
什么是DOI,文献DOI怎么找? 1336004
科研通“疑难数据库(出版商)”最低求助积分说明 637750
邀请新用户注册赠送积分活动 605820