Reduction of mitochondrial 3-oxoacyl-ACP synthase (OXSM) by hyperglycemia is associated with deficiency of α-lipoic acid synthetic pathway in kidney of diabetic mice

内分泌学 发病机制 内科学 糖尿病 硫辛酸 2型糖尿病 医学 生物 化学 生物化学 抗氧化剂
作者
Ting Gao,Shengnan Qian,Shuang Shen,Xiaoying Zhang,Junli Liu,Weiping Jia,Zhong Chen,Jianping Ye
出处
期刊:Biochemical and Biophysical Research Communications [Elsevier]
卷期号:512 (1): 106-111 被引量:16
标识
DOI:10.1016/j.bbrc.2019.02.155
摘要

LA (alpha-Lipoic acid) deficiency represents a risk factor in the pathogenesis of diabetic complications as synthetic LA is routinely used in the treatment of the complications in patients. The mechanism underlying LA deficiency remains elusive in the diabetic conditions. In the present study, we investigated the synthetic pathway of LA in both type 1 and 2 diabetic mice. LA deficiency was observed with a reduction in lipoylation of pyruvate dehydrogenase in the kidney of streptozocin-induced diabetic mice. Proteins of three enzymes (MCAT, OXSM and LIAS) in the LA synthetic pathway were examined in the kidney. A reduction was observed in OXSM, but not in the other two. In a 24h study in the cell culture, mRNA and protein of OXSM were transiently reduced by a high concentration of glucose (35 mM), and persistently decreased by TNF-α (20 nM). The high glucose effect was observed with the OXSM reduction in the kidney of db/db mice (type 2 diabetes model). The TNF-α effect was observed with OXSM reduction in the fat tissue of diet-induced obese mice. The result suggest that inhibition of OXSM by hyperglycemia and inflammation may contribute to the LA deficiency in the diabetic complications. The OXSM reduction suggests a new mechanism for the mitochondrial dysfunction in the pathogenesis of diabetic complications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
xianyuerkyt完成签到 ,获得积分10
刚刚
云氲完成签到 ,获得积分10
2秒前
5秒前
秋子david应助赵哥采纳,获得10
6秒前
hhhhh完成签到,获得积分10
8秒前
勤劳的亦巧完成签到 ,获得积分10
8秒前
ming完成签到,获得积分10
9秒前
9秒前
Szw666完成签到,获得积分10
9秒前
hhhhh发布了新的文献求助10
11秒前
紫羽完成签到,获得积分10
12秒前
17秒前
19秒前
21秒前
热血狂林应助lsy采纳,获得20
21秒前
现代青枫应助科研通管家采纳,获得20
21秒前
上官若男应助科研通管家采纳,获得10
21秒前
脑洞疼应助科研通管家采纳,获得10
21秒前
22秒前
NexusExplorer应助wenying采纳,获得10
22秒前
大模型应助科研通管家采纳,获得10
22秒前
星辰大海应助科研通管家采纳,获得10
22秒前
明亮的海冬完成签到,获得积分10
22秒前
FashionBoy应助科研通管家采纳,获得10
22秒前
酷波er应助科研通管家采纳,获得10
22秒前
wanci应助科研通管家采纳,获得10
22秒前
李爱国应助科研通管家采纳,获得10
22秒前
22秒前
爆米花应助科研通管家采纳,获得10
22秒前
我是老大应助科研通管家采纳,获得10
22秒前
22秒前
24秒前
科研通AI2S应助bill采纳,获得10
27秒前
rosalieshi应助bill采纳,获得30
27秒前
8R60d8应助bill采纳,获得10
27秒前
科研通AI2S应助bill采纳,获得30
27秒前
andrele应助bill采纳,获得10
27秒前
科研通AI2S应助bill采纳,获得10
27秒前
科研通AI2S应助bill采纳,获得10
27秒前
李大山应助bill采纳,获得10
27秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
安全防范技术与工程 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3190847
求助须知:如何正确求助?哪些是违规求助? 2840059
关于积分的说明 8026781
捐赠科研通 2503288
什么是DOI,文献DOI怎么找? 1336823
科研通“疑难数据库(出版商)”最低求助积分说明 637964
邀请新用户注册赠送积分活动 606279