Reduction of mitochondrial 3-oxoacyl-ACP synthase (OXSM) by hyperglycemia is associated with deficiency of α-lipoic acid synthetic pathway in kidney of diabetic mice

内分泌学 发病机制 内科学 糖尿病 硫辛酸 2型糖尿病 医学 生物 化学 生物化学 抗氧化剂
作者
Ting Gao,Shengnan Qian,Shuang Shen,Xiaoying Zhang,Junli Liu,Weiping Jia,Zhong Chen,Jianping Ye
出处
期刊:Biochemical and Biophysical Research Communications [Elsevier]
卷期号:512 (1): 106-111 被引量:16
标识
DOI:10.1016/j.bbrc.2019.02.155
摘要

LA (alpha-Lipoic acid) deficiency represents a risk factor in the pathogenesis of diabetic complications as synthetic LA is routinely used in the treatment of the complications in patients. The mechanism underlying LA deficiency remains elusive in the diabetic conditions. In the present study, we investigated the synthetic pathway of LA in both type 1 and 2 diabetic mice. LA deficiency was observed with a reduction in lipoylation of pyruvate dehydrogenase in the kidney of streptozocin-induced diabetic mice. Proteins of three enzymes (MCAT, OXSM and LIAS) in the LA synthetic pathway were examined in the kidney. A reduction was observed in OXSM, but not in the other two. In a 24h study in the cell culture, mRNA and protein of OXSM were transiently reduced by a high concentration of glucose (35 mM), and persistently decreased by TNF-α (20 nM). The high glucose effect was observed with the OXSM reduction in the kidney of db/db mice (type 2 diabetes model). The TNF-α effect was observed with OXSM reduction in the fat tissue of diet-induced obese mice. The result suggest that inhibition of OXSM by hyperglycemia and inflammation may contribute to the LA deficiency in the diabetic complications. The OXSM reduction suggests a new mechanism for the mitochondrial dysfunction in the pathogenesis of diabetic complications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
华仔应助xy采纳,获得10
1秒前
3秒前
席玲完成签到,获得积分10
3秒前
4秒前
4秒前
李健应助教授王采纳,获得30
4秒前
小贾完成签到,获得积分10
4秒前
小蘑菇应助石榴采纳,获得10
4秒前
八九完成签到 ,获得积分10
4秒前
6秒前
田様应助for_abSCI采纳,获得10
6秒前
我爱夏天发布了新的文献求助10
7秒前
May_9527完成签到,获得积分10
7秒前
7秒前
玥月完成签到 ,获得积分10
8秒前
懒羊羊完成签到 ,获得积分10
8秒前
烟花应助hshsh采纳,获得10
8秒前
8秒前
111完成签到,获得积分10
10秒前
参商发布了新的文献求助10
10秒前
十年发布了新的文献求助10
10秒前
10秒前
呜啦啦发布了新的文献求助10
10秒前
11秒前
WANGJIA关注了科研通微信公众号
12秒前
12秒前
precious完成签到 ,获得积分10
13秒前
谦让蜜蜂完成签到,获得积分10
13秒前
xhl发布了新的文献求助10
13秒前
JamesPei应助queer采纳,获得10
14秒前
醍醐不醒完成签到,获得积分10
14秒前
15秒前
stephen完成签到,获得积分10
15秒前
15秒前
16秒前
昂首完成签到,获得积分20
16秒前
17秒前
Hannipham完成签到,获得积分10
17秒前
烂漫的松完成签到,获得积分10
18秒前
19秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3181250
求助须知:如何正确求助?哪些是违规求助? 2831509
关于积分的说明 7985112
捐赠科研通 2493502
什么是DOI,文献DOI怎么找? 1330163
科研通“疑难数据库(出版商)”最低求助积分说明 635934
版权声明 602955