化学
解吸电喷雾电离
生物分子
质谱法
电喷雾电离
质谱成像
电离
离子
色谱法
分析化学(期刊)
解吸
化学电离
有机化学
吸附
生物化学
作者
Chengyuan Liu,Keke Qi,Lei Yao,Ying Xiong,Xuan Zhang,Jianye Zang,Changlin Tian,Minggao Xu,Jiuzhong Yang,Zhenkun Lin,Yongmei Lv,Wei Xiong,Yang Pan
标识
DOI:10.1021/acs.analchem.9b00520
摘要
Desorption electrospray ionization (DESI) mass spectrometry imaging (MSI) can simultaneously record the 2D distribution of polar biomolecules in tissue slices at ambient conditions. However, sensitivity of DESI-MSI for nonpolar compounds is restricted by low ionization efficiency and strong ion suppression. In this study, a compact postphotoionization assembly combined with DESI (DESI/PI) was developed for imaging polar and nonpolar molecules in tissue sections by switching off/on a portable krypton lamp. Compared with DESI, higher signal intensities of nonpolar compounds could be detected with DESI/PI. To further increase the ionization efficiency and transport of charged ions of DESI/PI, the desorption solvent composition and gas flow in the ionization tube were optimized. In mouse brain tissue, more than 2 orders of magnitude higher signal intensities for certain neutral biomolecules like creatine, cholesterol, and GalCer lipids were obtained by DESI/PI in the positive ion mode, compared with that of DESI. In the negative ion mode, ion yields of DESI/PI for glutamine and some lipids (HexCer, PE, and PE-O) were also increased by several-fold. Moreover, nonpolar constituents in plant tissue, such as catechins in leaf shoots of tea, could also be visualized by DESI/PI. Our results indicate that DESI/PI can expand the application field of DESI to nonpolar molecules, which is important for comprehensive imaging of biomolecules in biological tissues with moderate spatial resolution at ambient conditions.
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