生物
基因组
DNA
基因组DNA
计算生物学
DNA纳米球测序
DNA测序
基因组学
人类基因组
DNA–DNA杂交
遗传学
基因组文库
基因
基序列
作者
Nathaniel Wand,Deb Smith,Andrew A. Wilkinson,Ashleigh E Rushton,Stephen J. W. Busby,Iain B. Styles,Robert K. Neely
摘要
Abstract We report an approach for visualizing DNA sequence and using these ‘DNA barcodes’ to search complex mixtures of genomic material for DNA molecules of interest. We demonstrate three applications of this methodology; identifying specific molecules of interest from a dataset containing gigabasepairs of genome; identification of a bacterium from such a dataset and, finally, by locating infecting virus molecules in a background of human genomic material. As a result of the dense fluorescent labelling of the DNA, individual barcodes of the order 40 kb pairs in length can be reliably identified. This means DNA can be prepared for imaging using standard handling and purification techniques. The recorded dataset provides stable physical and electronic records of the total genomic content of a sample that can be readily searched for a molecule or region of interest.
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