酵母
转化(遗传学)
转化效率
双杂交筛选
领域(数学分析)
生物
遗传学
基因
数学
农杆菌
数学分析
作者
John S. Reece‐Hoyes,Albertha J.M. Walhout
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2018-07-01
卷期号:2018 (7): pdb.prot094995-pdb.prot094995
被引量:7
标识
DOI:10.1101/pdb.prot094995
摘要
High-efficiency yeast transformation is used for integrations into YM4271 (yeast one-hybrid (Y1H) DNA-bait generation), for transforming libraries of activation domain (AD)-prey clones into Y1H and yeast two-hybrid (Y2H)-bait strains, and for gap repair. The protocol takes 2 d to complete.
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