质谱法
质谱成像
马尔迪成像
化学
解吸
基质辅助激光解吸/电离
色谱法
吸附
有机化学
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 7-14
被引量:18
标识
DOI:10.1007/978-1-4939-7051-3_2
摘要
Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry Imaging (MSI) is fast becoming an industry leading technique as a means of investigating the distributions of protein and peptide molecules directly from sections of tissue. Developing protocols for the analysis of FFPE tissue opens up numerous opportunities for novel biomarker discovery, due to the large number of tissue banks containing FFPE biopsy samples. This chapter reports the analysis of both fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues using such protocols.
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