生物
转录组
RNA序列
多样性(政治)
细胞生物学
基因表达
核糖核酸
计算生物学
基因
细胞
遗传学
人类学
社会学
作者
Renchao Chen,Xiaoji Wu,Lan Jiang,Yi Zhang
出处
期刊:Cell Reports
[Elsevier]
日期:2017-03-01
卷期号:18 (13): 3227-3241
被引量:529
标识
DOI:10.1016/j.celrep.2017.03.004
摘要
Highlights•Transcriptome-based cell-type classification in mouse adult hypothalamus•Transcriptional features of oligodendrocyte differentiation and tanycyte subtypes•Neuropeptide and receptor expression patterns of hypothalamic neuronal subtypes•Cell-type-specific transcriptional responses to food deprivationSummaryThe hypothalamus is one of the most complex brain structures involved in homeostatic regulation. Defining cell composition and identifying cell-type-specific transcriptional features of the hypothalamus is essential for understanding its functions and related disorders. Here, we report single-cell RNA sequencing results of adult mouse hypothalamus, which defines 11 non-neuronal and 34 neuronal cell clusters with distinct transcriptional signatures. Analyses of cell-type-specific transcriptomes reveal gene expression dynamics underlying oligodendrocyte differentiation and tanycyte subtypes. Additionally, data analysis provides a comprehensive view of neuropeptide expression across hypothalamic neuronal subtypes and uncover Crabp1+ and Pax6+ neuronal populations in specific hypothalamic sub-regions. Furthermore, we found food deprivation exhibited differential transcriptional effects among the different neuronal subtypes, suggesting functional specification of various neuronal subtypes. Thus, the work provides a comprehensive transcriptional perspective of adult hypothalamus, which serves as a valuable resource for dissecting cell-type-specific functions of this complex brain region.Graphical abstract
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