An experimental animal model of yellowfin seabream (Acanthopagrus latus) for Amyloodinium ocellatum infection

生物 动物
作者
Zhicheng Li,Zhihong Zhong,Jingyu Zhuang,Luo Zhi,Qing Han,Jizhen Cao,Baotun Wang,An‐Xing Li
出处
期刊:Aquaculture [Elsevier]
卷期号:574: 739641-739641 被引量:6
标识
DOI:10.1016/j.aquaculture.2023.739641
摘要

Amyloodinium ocellatum is a worldwide distributed protozoan that infects almost all marine fish species, causing fatal amyloodiniosis. However, highly effective antiparasitic agents for controlling amyloodiniosis are lacking. This study was conducted to establish a laboratory model of A. ocellatum infection, facilitating the development of alternative treatment options and immunostimulants for A. ocellatum using yellowfin seabream (Acanthopagrus latus) as the experimental host. The life cycle and preservation strategies of A. ocellatum, the stability of dinospore infection in A. latus, and the tolerance of A. latus to A. ocellatum infection were observed and evaluated. Under suitable conditions (temperature: 27 ± 1 °C; salinity: 30 ± 1‰), A. ocellatum completes its life cycle on A. latus in 4–5 d and peak trophont detachment from A. latus occurs at 44–52 h post-infection. The peak period when tomonts release dinospores occurs 51–60 h after incubation at 28 °C. Dinospores have high vitality and infectivity within 6 h of release from tomonts. The infection model developed in this study is stable, with a 39.85–44.68% dinospore infection rate. Additionally, gills were the main A. ocellatum infection site, accounting for 70.49–72.68% of the total trophonts in infected A. latus. The maximal tolerance dose (MTD) and lethal concentration 50 (LC50) of A. ocellatum to A. latus were 14,686 and 28,901 dinospores per fish, respectively. Tomonts can be preserved for at least 3 months at 12 °C. This study established an A. ocellatum infection model under laboratory conditions, which is potentially helpful in exploring alternative treatments and investigating the pathogenic mechanisms of amyloodiniosis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
1秒前
葡萄成熟应助国产耗材采纳,获得10
1秒前
大个应助小垃圾采纳,获得10
1秒前
2秒前
aaashirz_完成签到,获得积分10
2秒前
3秒前
soar发布了新的文献求助10
3秒前
贺小刚完成签到,获得积分10
3秒前
丘比特应助小陈栗子采纳,获得10
5秒前
Silence完成签到,获得积分10
5秒前
Lin完成签到,获得积分10
5秒前
7秒前
7秒前
CH应助超帅涔采纳,获得10
8秒前
老迟到的念双完成签到,获得积分10
8秒前
酷酷羊完成签到,获得积分10
9秒前
香翔想相完成签到,获得积分10
9秒前
jw完成签到,获得积分10
9秒前
研友_VZG7GZ应助害羞含雁采纳,获得10
10秒前
10秒前
htc2022完成签到,获得积分10
10秒前
jw发布了新的文献求助10
12秒前
13秒前
14秒前
彩色迎丝完成签到 ,获得积分20
14秒前
yyhgyg发布了新的文献求助10
14秒前
yaoyh_gc发布了新的文献求助10
14秒前
风中的博完成签到 ,获得积分10
15秒前
国产耗材完成签到,获得积分10
16秒前
Colin_chen发布了新的文献求助10
16秒前
17秒前
深情安青应助风趣绯采纳,获得10
18秒前
18秒前
天天完成签到 ,获得积分10
18秒前
李爱国应助否定的否定采纳,获得10
19秒前
19秒前
20秒前
彩色迎丝关注了科研通微信公众号
21秒前
21秒前
高分求助中
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
Дружба 友好报 (1957-1958) 1000
The Data Economy: Tools and Applications 1000
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufe 700
A Dissection Guide & Atlas to the Rabbit 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3101245
求助须知:如何正确求助?哪些是违规求助? 2752689
关于积分的说明 7620005
捐赠科研通 2404773
什么是DOI,文献DOI怎么找? 1275998
科研通“疑难数据库(出版商)”最低求助积分说明 616673
版权声明 599058