亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

The neuroprotective mechanisms of naringenin: Inhibition of apoptosis through the PI3K/AKT pathway after hypoxic-ischemic brain damage

神经保护 柚皮素 药理学 神经红蛋白 生物 PI3K/AKT/mTOR通路 标记法 免疫印迹 细胞凋亡 生物化学 抗氧化剂 类黄酮 基因 珠蛋白
作者
Luyao Li,Zhen Lin,Junhui Yuan,Pingping Li,Qi Wang,Namki Cho,Yi Wang,Zhenlang Lin
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:318: 116941-116941 被引量:40
标识
DOI:10.1016/j.jep.2023.116941
摘要

Naringenin (NGN) is a widely distributed flavonoid with potent antioxidant and neuroprotective properties. Neuroprotective agents play a crucial role in the treatment of hypoxic-ischemic encephalopathy (HIE). It has shown potential therapeutic effects for neurological disorders. However, its efficacy on HIE is yet to be investigated.This study aims to investigate the potential neuroprotective effect of naringenin and its underlying molecular mechanisms in reducing oxidative stress, apoptosis, and improving brain outcomes following HIE. Additionally, the study aims to identify the potential targets, mechanisms, and functions of naringenin using network pharmacology analysis.Neonatal mice were exposed to the hypoxic-ischemic brain damage (HIBD) model to determine brain water content, and brain tissue was subjected to hematoxylin and eosin (HE), immunohistochemistry (IHC), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and Nissl staining to investigate its neuroprotective effects. Furthermore, the neonatal mouse primary neuron oxygen-glucose deprivation (OGD) model to measure reactive oxygen species (ROS) production in vitro. The protein levels were characterized by Western Blot, and mRNA levels were evaluated by a real-time quantitative PCR detecting system (qPCR). Transmission electron microscopy (TEM) and mitochondrial fluorescent staining were used to observe mitochondrial morphology. Neuronal nuclei (NeuN) and microtubule-associated protein 2 (MAP2) were detected by Immunofluorescence (IF). Finally, network pharmacology was employed to determine the common target of naringenin and HIE. The core genes were obtained via protein-protein interaction networks (PPI) analysis and molecular docking was examined, and the mechanism of action was explored through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Additionally, small interfering RNA (siRNA) was constructed for verification.Naringenin has a neuroprotective effect in HIBD by modulating Vegfa expression and activating the PI3K/AKT pathway to inhibit apoptosis. Furthermore, molecular docking results suggest that Vegfa is a potential binding target of naringenin, and silencing Vegfa partially reverses the pharmacological effects of NGN.Our findings suggest that naringenin demonstrates potential clinical application for treating HIE as a novel neuroprotective agent.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科研通AI5应助sljzhangbiao11采纳,获得10
4秒前
又来注水了完成签到,获得积分10
15秒前
快乐海豚完成签到 ,获得积分10
21秒前
科研通AI5应助科研通管家采纳,获得10
25秒前
科研狗的春天完成签到 ,获得积分10
28秒前
Lucas应助董可以采纳,获得10
35秒前
41秒前
Aqib发布了新的文献求助10
46秒前
47秒前
科研通AI5应助西瓜嘻嘻嘻采纳,获得10
50秒前
吴昕昕发布了新的文献求助10
52秒前
未晚完成签到 ,获得积分10
54秒前
香蕉觅云应助Aqib采纳,获得10
58秒前
早晨发布了新的文献求助10
1分钟前
孤鸿.完成签到 ,获得积分10
1分钟前
1分钟前
彭于晏应助11采纳,获得10
1分钟前
1分钟前
吴昕昕完成签到,获得积分10
1分钟前
1分钟前
iwaking完成签到,获得积分10
1分钟前
hxn发布了新的文献求助30
1分钟前
通通发布了新的文献求助10
1分钟前
早晨完成签到,获得积分10
1分钟前
Lucas应助通通采纳,获得10
1分钟前
1分钟前
WANG完成签到,获得积分10
1分钟前
有机发布了新的文献求助10
1分钟前
摆渡人完成签到,获得积分10
1分钟前
提桶跑路完成签到 ,获得积分10
1分钟前
温柔的天奇完成签到 ,获得积分10
1分钟前
实验耗材完成签到 ,获得积分10
1分钟前
1分钟前
lhlhl完成签到,获得积分10
1分钟前
1分钟前
2分钟前
小小林发布了新的文献求助10
2分钟前
zl13332完成签到 ,获得积分10
2分钟前
2分钟前
通通完成签到 ,获得积分10
2分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990020
求助须知:如何正确求助?哪些是违规求助? 3532077
关于积分的说明 11256276
捐赠科研通 3270943
什么是DOI,文献DOI怎么找? 1805139
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228