Surface hydrophilicity-mediated migration of nano/microparticles under temperature gradient in a confined space

Particle transport by a temperature gradient is prospective in many biomedical applications. However, the prevalence of boundary confinement in practical use introduces synergistic effects of thermophoresis and thermo-osmosis, causing controversial phenomena and great difficulty in understanding the mechanisms.We developed a microfluidic chip with a uniform temperature gradient and switchable substrate hydrophilicity to measure the migrations of various particles (d = 200 nm - 2 μm), through which the effects of particle thermophoresis and thermo-osmotic flow from the substrate surface were decoupled. The contribution of substrate hydrophilicity on thermo-osmosis was examined. Thermophoresis was measured to clarify its dependence on particle size and hydrophilicity.This paper reports the first experimental evidence of a large enthalpy-dependent thermo-osmotic mobility χ ∼ ΔH on a hydrophobic polymer surface, which is 1-2 orders of magnitude larger than that on hydrophilic surfaces. The normalized Soret coefficient for polystyrene particles, ST/d = 18.0 K-1µm-1, is confirmed to be constant, which helps clarify the controversy of the size dependence. Besides, the Soret coefficient of hydrophobic proteins is approximately-four times larger than that of hydrophilic extracellular vesicles. These findings suggest that the intrinsic slip on the hydrophobic surface could enhance both surface thermo-osmosis and particle thermophoresis.