Soybean (Glycine max L. Merrill.) crop is affected by number of pathogenic fungi, which are mostly seed borne, causing significant qualitative and quantitative losses. Therefore, present In vitro study was conducted to asses efficacy of fungicides against three major pathogenic seed borne fungi viz., Fusarium verticillioides, Macrophomina phaseolina and Alternaria alternata, by applying Poisoned Food Technique. Three separate experiments were planned and conducted with Completely Randomized Design (CRD) and all the treatments replicated thrice. The results revealed that all the seven seed dressing fungicides significantly inhibited mycelial growth of Fusarium verticillioides, Macrophomina phaseolina and Alternaria alternata, over untreated control. However, the fungicides viz., tebuconazole 25% WG and carbendazim 12% + mancozeb 63% 75 WP were found most effective with 100 per cent mycelial growth inhibition of Fusarium verticillioides and rest of the fungicides tested were comparatively less effective. However, mancozeb 75% WP, carboxin 37.5% + thiram 37.5% 75 WP and carbendazim 12% + mancozeb 63% 75 WP were found most effective with 100 per cent mycelial growth inhibition of Macrophomina phaseolina and rest of the fungicides tested were comparatively less effective. However, tebuconazole 25% WG and carboxin 37.5% + thiram 37.5% 75 WP were found most effective with 38.52 and 85.93%, respectively mycelial growth inhibition of Alternaria alternata and rest of the fungicides tested were comparatively less effective.