化学
荧光团
过氧亚硝酸盐
荧光
体内
生物物理学
活性氧
糖尿病
生物化学
超氧化物
内分泌学
酶
光学
物理
生物技术
生物
医学
作者
Ya‐Jing Shang,Yuanyuan Li,Jialing Hu,Yiru Wang,Shengtao Li,Xing-Hu Wu,Aihong Zhang,Yu-Wei Tan,Ting Liang,Xiaobo Zhu,Yan‐Fei Kang
标识
DOI:10.1016/j.aca.2024.342817
摘要
Diabetes has become one of the most common endocrine and metabolic diseases threatening human health, which can induce mitochondrial dysfunction and exacerbate the excessive production of reactive oxygen species (ROS). Among them, ONOO- level fluctuation was closely related to diabetes. Hence, it is of great significance to develop a near-infrared fluorescence probe for visualizing ONOO- level fluctuations in diabetes. In this paper, we constructed a fluorescence probe YBL with dicyano-isophorone derivative as fluorophore and diphenyl phosphate as ONOO- response site, which can detect ONOO- with the low detection limit (39.8 nM) and exhibit excellent selectivity and sensitivity. The probe YBL has been applied to monitor intracellular ONOO- level fluctuations. Meanwhile, the image results showed that high sugar promoted the increase of ONOO- level in cells. More important, the probe YBL can be used for imaging in mice, and the results showed that content of ONOO- was increased in diabetic mice. Therefore, the probe YBL provided a tool for understanding diabetes progression by imaging ONOO-.
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