单克隆抗体
受体
淋巴细胞
分子生物学
T淋巴细胞
胸苷
DNA合成
生物
细胞表面受体
T细胞
单克隆
抗体
刀豆蛋白A
丝裂原活化蛋白激酶
细胞培养
免疫学
抗原
DNA
生物化学
体外
免疫系统
遗传学
作者
J P Van Wauwe,J R De Mey,Jan Goossens
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:1980-06-01
卷期号:124 (6): 2708-2713
被引量:503
标识
DOI:10.4049/jimmunol.124.6.2708
摘要
Abstract OKT3, a monoclonal anti-human T cell antibody (IgG2), was found to induce DNA synthesis in human peripheral lymphocyte cultures. OKT3 induced maximal mitogenesis at a concentration of 10 to 20 ng/ml and was about 20-fold more potent than PHA as a mitogen. No high-dose inhibition of thymidine incorporation was noticed at concentrations up to 2.5 microgram OKT3/ml. The monovalent Fab fragment of OKT3 was also mitogenic but about 100 times less potent than the parent IgG. OKT3 appeared to be a T lymphocyte mitogen as only sheep red blood cell rosetting lymphocytes were responsive. Quantitative studies on the binding of 125I-labeled Fab fragment of OKT3 to human lymphocytes showed an average of 5.1 x 10(4) receptor sites/cell with an association of about 10(8) M-1 at 37 degrees C, with no heterogeneity of the cell binding sites. These data suggest a strong interaction of the monoclonal OKT3 with a limited number of identical T cell membrane receptors. As this interaction can trigger mitogenesis, the cell membrane determinant recognized by OKT3 could be described as a "T cell stimulation receptor." The mitogenecity of the lymphocytes is not solely dependent on cross-linking of these receptors.
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