Comprehensive analysis of endoplasmic reticulum stress-associated genes signature of ulcerative colitis

小桶 基因 基因表达 生物 基因表达谱 计算生物学 内质网 遗传学 转录组
作者
Beiying Deng,Fei Liao,Yinghui Liu,Pengzhan He,Shuchun Wei,Chuan Liu,Weiguo Dong
出处
期刊:Frontiers in Immunology [Frontiers Media]
卷期号:14 被引量:8
标识
DOI:10.3389/fimmu.2023.1158648
摘要

Background Endoplasmic reticulum stress (ERS) is a critical factor in the development of ulcerative colitis (UC); however, the underlying molecular mechanisms remain unclear. This study aims to identify pivotal molecular mechanisms related to ERS in UC pathogenesis and provide novel therapeutic targets for UC. Methods Colon tissue gene expression profiles and clinical information of UC patients and healthy controls were obtained from the Gene Expression Omnibus (GEO) database, and the ERS-related gene set was downloaded from GeneCards for analysis. Weighted gene co-expression network analysis (WGCNA) and differential expression analysis were utilized to identify pivotal modules and genes associated with UC. A consensus clustering algorithm was used to classify UC patients. The CIBERSORT algorithm was employed to evaluate the immune cell infiltration. Gene Set Variation Analysis (GSVA), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to explore potential biological mechanisms. The external sets were used to validate and identify the relationship of ERS-related genes with biologics. Small molecule compounds were predicted using the Connectivity Map (CMap) database. Molecular docking was performed to simulate the binding conformation of small molecule compounds and key targets. Results The study identified 915 differentially expressed genes (DEGs) and 11 ERS-related genes (ERSRGs) from the colonic mucosa of UC patients and healthy controls, and these genes had good diagnostic value and were highly correlated. Five potential small-molecule drugs sharing tubulin inhibitors were identified, including albendazole, fenbendazole, flubendazole, griseofulvin, and noscapine, among which noscapine exhibited the highest correlation with a high binding affinity to the targets. Active UC and 10 ERSRGs were associated with a large number of immune cells, and ERS was also associated with colon mucosal invasion of active UC. Significant differences in gene expression patterns and immune cell infiltration abundance were observed among ERS-related subtypes. Conclusion The results suggest that ERS plays a vital role in UC pathogenesis, and noscapine may be a promising therapeutic agent for UC by affecting ERS.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
猪猪hero发布了新的文献求助10
刚刚
打打应助哈哈鹿采纳,获得10
1秒前
skye完成签到,获得积分10
1秒前
CipherSage应助义气如萱采纳,获得10
1秒前
zty发布了新的文献求助30
1秒前
SS发布了新的文献求助10
1秒前
戴岱发布了新的文献求助10
3秒前
5秒前
量子星尘发布了新的文献求助10
7秒前
8秒前
张大英发布了新的文献求助10
10秒前
马er完成签到,获得积分20
10秒前
奮斗完成签到,获得积分10
10秒前
戴岱完成签到,获得积分10
11秒前
哈哈鹿发布了新的文献求助10
12秒前
12秒前
黎明森完成签到,获得积分10
12秒前
Monica应助朴素若枫采纳,获得30
13秒前
14秒前
科研通AI5应助科研通管家采纳,获得10
14秒前
SYLH应助科研通管家采纳,获得30
14秒前
14秒前
czh应助科研通管家采纳,获得10
14秒前
科研通AI2S应助科研通管家采纳,获得10
14秒前
14秒前
今后应助科研通管家采纳,获得10
14秒前
顾矜应助科研通管家采纳,获得10
14秒前
14秒前
丘比特应助科研通管家采纳,获得10
14秒前
赘婿应助科研通管家采纳,获得10
14秒前
14秒前
14秒前
14秒前
王羊补牢发布了新的文献求助10
17秒前
布施德完成签到 ,获得积分10
18秒前
Zzz完成签到,获得积分10
19秒前
呆萌板凳完成签到,获得积分20
21秒前
22秒前
23秒前
哈哈鹿完成签到,获得积分10
23秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989242
求助须知:如何正确求助?哪些是违规求助? 3531393
关于积分的说明 11253753
捐赠科研通 3270010
什么是DOI,文献DOI怎么找? 1804868
邀请新用户注册赠送积分活动 882084
科研通“疑难数据库(出版商)”最低求助积分说明 809136