化学
过氧亚硝酸盐
内生
戒毒(替代医学)
溶酶体
检出限
氧化应激
荧光
免疫系统
生物化学
酶
免疫学
病理
色谱法
生物
医学
替代医学
超氧化物
物理
量子力学
作者
Xiao Wang,Xuechuan Wang,Zhongxue Bai,Kaiqiang Du,Junli Zhang,Qingxin Han
出处
期刊:Talanta
[Elsevier]
日期:2023-12-26
卷期号:270: 125581-125581
被引量:7
标识
DOI:10.1016/j.talanta.2023.125581
摘要
The liver plays a crucial role in several important processes in the human body, including metabolism, detoxification, and immune function. When the liver experiences acute injury, it can cause significant harm and requires prompt detection. Traditional biomarkers lack specificity and cannot detect changes in real-time, making them unsuitable for monitoring pathological processes. Recent studies have shown that acute liver injury (ALI) is closely related to oxidative stress, with peroxynitrite (ONOO−) being a vital byproduct of liver metabolism and become a critical biomarker for detecting liver damage. As a result, this research developed an activatable near-infrared fluorescent probe W-3a that can be used to detect endogenous ONOO− in a mouse model of ALI induced by lipopolysaccharides (LPS). The probe has high selectivity and anti-interference ability, with a reaction time <10 min and a detection limit of 85 nM. It was successfully utilized in detecting endogenous ONOO− in cells and live imaging of ALI mice.
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