生物
蛋白质亚单位
表位
病毒学
高尔基体
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
抗体
病毒
构象变化
细胞生物学
2019年冠状病毒病(COVID-19)
生物物理学
生物化学
遗传学
内质网
医学
疾病
病理
基因
传染病(医学专业)
作者
Yanjun Wu,Soak Kuan Lai,Conrad En-Zuo Chan,Boon Huan Tan,Richard J. Sugrue
出处
期刊:Virology
[Elsevier]
日期:2024-07-01
卷期号:: 110187-110187
标识
DOI:10.1016/j.virol.2024.110187
摘要
Recombinant SARS-CoV-2 S protein expression was examined in Vero cells by imaging using the human monoclonal antibody panel (PD4, PD5, sc23, and sc29). The PD4 and sc29 antibodies recognised conformational specific epitopes in the S2 protein subunit at the Endoplasmic reticulum and Golgi complex. While PD5 and sc23 detected conformationally specific epitopes in the S1 protein subunit at the Golgi complex, only PD5 recognised the receptor binding domain (RBD). A comparison of the staining patterns of PD5 with non-conformationally specific antibodies that recognises the S1 subunit and RBD suggested the PD5 recognised a conformational structure within the S1 protein subunit. Our data suggests the antibody binding epitopes recognised by the human monoclonal antibodies formed at different locations in the secretory pathway during S protein transport, but a conformational change in the S1 protein subunit at the Golgi complex formed antibody binding epitopes that are recognised by virus neutralising antibodies.
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