Fluorescent Sensor/Tracker for Biocompatible and Real-time Monitoring of Ultra-trace Arsenic Toxicants in Living Cells

Real-time monitoring and tracking of extreme toxins that penetrate into living cells by using biocompatible, low-cost visual detection via fluorescent monitors are vitally essential to reduce health hazards. Herein, we report a simple engineering design of biocompatible and fluorescent sensors/trackers for real-time monitoring and ultra-trace tracking (up to ppb) of extremely toxic substances (such as arsenic species) in living cells. The biocompatible As(V) sensor (BAS) design is fabricated via successful dressing/decoration process of 2-hydroxy 5-methyl isophthalaldehyde fluorescent receptor into hierarchical organic-inorganic carriers that have micro-hollow geodes, swirled caves and nest-shaped cages, and uniform cubic structures. The BAS monitors show evidence for the selective trapping/detecting/tracking of As(V) species in biological cells (i.e., HeLa cells) despite the coexistence of highly competitive and interfered species. Our simple batch-contact sensing assays shows real-space evidence of the continuous monitoring of As(V) species in HeLa cells with ultra-sensitive detection (i.e., with a low detection limit of 0.149 ppb) and rapid recognition (i.e., in the order of seconds). Significantly, the BAS monitors did not affect the cell population and achieved low cytotoxicity and high cell viability during the monitoring/tracking process inside HeLa cells. The high biocompatibility of BAS remarkably allows precise quantification and real-time monitoring/tracking of toxicant targets in living cells.