大肠杆菌
质粒
基因
遗传学
对偶(语法数字)
生物
DNA转座因子
大肠杆菌蛋白质类
肠杆菌科
计算生物学
转座因子
基因组
艺术
文学类
作者
Menghui Liu,Wei Ge,Guomei Zhong,Yuqing Yang,Luying Xun,Yongzhen Xia
标识
DOI:10.1021/acssynbio.4c00140
摘要
The efficiency of valuable metabolite production by engineered microorganisms underscores the importance of stable and controllable gene expression. While plasmid-based methods offer flexibility, integrating genes into host chromosomes can establish stability without selection pressure. However, achieving site-directed multicopy integration presents challenges, including site selection and stability. We introduced a stable multicopy integration method by using a novel dual-plasmid mini-Tn5 system to insert genes into
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