生物
16S核糖体RNA
二氨基酸
根际
阿拉伯糖
拉伤
放线菌门
微生物学
植物
细菌
生物化学
基因
遗传学
发酵
解剖
基因序列
木糖
作者
Xu Yan,Xue Zhao,Jiaxin Jin,Ran Zhang,Changjian Zhou,Zishan Wang,Siqi Yao,Xiangjing Wang,Wensheng Xiang,Jia Song
标识
DOI:10.1099/ijsem.0.006559
摘要
A novel protease-producing and cellulose-degrading actinobacterium, designated strain NEAU-NG30 T , was isolated from a melon rhizosphere soil sample collected in Harbin, Heilongjiang Province, China, and established its status using a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-NG30 T was closely related to Amycolatopsis bullii DSM 45802 T (98.7%) and Amycolatopsis vancoresmycina DSM 44592 T (98.3%). The phospholipid profile contained diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylinositol. The diagnostic sugars in cell hydrolysates were determined to be galactose and arabinose. Cell walls contained meso -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-9(H 4 ). The major fatty acids were iso-C 15:0 and iso-C 16:0 . Meanwhile, genome analysis of sequences revealed a genome size of 9 338 250 bp and a DNA G+C content of 71.6%. In addition, the average nucleotide identity values and the level of digital DNA–DNA hybridization between strain NEAU-NG30 T and its reference strains fall below the thresholds typically used for delineating prokaryote species. According to phenotypic, chemotaxonomic and genotypic studies, it is indicated that strain NEAU-NG30 T is considered to be a novel species of the genus Amycolatopsis , for which the name Amycolatopsis melonis sp. nov. is proposed, with NEAU-NG30 T (=MCCC 1K08677 T =JCM 35654 T ) as the type strain.
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