清脆的
体内
协议(科学)
生物
细胞生物学
计算生物学
化学
医学
遗传学
基因
病理
替代医学
作者
Yuki Noguchi,Masahiro Maruoka,Jun Suzuki
出处
期刊:STAR protocols
[Elsevier]
日期:2024-09-01
卷期号:5 (3): 103306-103306
标识
DOI:10.1016/j.xpro.2024.103306
摘要
In vivo genome-wide screening elucidates tissue-specific molecular events. Here, we present a protocol for an in vivo genome-wide CRISPR-Cas9 single-guide RNA (sgRNA) library screening technique optimized for mouse testicular cells to investigate spermatogenesis. We describe steps for virus injection, sperm sorting, and primase-based whole-genome amplification. We then detail procedures for library reconstruction using a "revival screening" technique. Our approach reveals intricate spermatogenesis processes and is adaptable for diverse tissue-specific studies. For complete details on the use and execution of this protocol, please refer to Noguchi et al.
科研通智能强力驱动
Strongly Powered by AbleSci AI