Strain sensor films are widely applied in electronic skins, soft robotics and human health monitoring etc. However, fabrication of ultrasensitive and highly stretchable strain sensor films remains a great challenge because of the trade-off between sensitivity and stretchability. Here, we report an aligned silver nanowires (Ag NWs)/polymer composite film prepared by a float assembly method. After perpendicularly stacking two layers of Ag NWs/polymer composite films into one sensor film, it exhibits an ultrahigh sensitivity with gauge factor (GF) up to 2.98 × 106 and a very large working range of 200% due to different resistance response mechanism of the cross film structure. The low limit of detection is as low as 0.001%. Accordingly, the strain sensor films can detect both large deformation and subtle vibration for human motion and monitoring, even early warning of earthquakes. This work provides a new idea to fabricate stain sensor films with both ultrahigh sensitivity and large stretchability.