Study on Mechanisms of Resistance to SDHI Fungicide Pydiflumetofen in Fusarium fujikuroi

Rice bakaenii disease (RBD) is a widespread and devastating disease mainly caused by Fusarium fujikuroi. Pydiflumetofen (Pyd) is a novel succinate dehydrogenase inhibitor (SDHI) with strong inhibitory activity against F. fujikuroi, but the mechanism of resistance to Pyd has not been well studied for this pathogen. Through fungicide adaption, a total of 12 Pyd-resistant mutants were obtained and the resistance level could be divided into three categories of high resistance (HR), moderate resistance (MR), and low resistance (LR) with resistance factors (RF) of 184.04–672.90, 12.63–42.49, and <10, respectively. Seven genotypes of point mutations in FfSdh genes (FfSdhBH248L, FfSdhBH248D, FfSdhBH248Y, FfSdhC2A83V, FfSdhC2H144Y, FfSdhDS106F, and FfSdhDE166K) were found in these mutants, among which genotype FfSdhBH248L and FfSdhC2A83V mutants showed HR, genotype FfSdhBH248D, FfSdhBH248Y, FfSdhC2H144Y, and FfSdhDE166K mutants showed MR, and genotype FfSdhDS106F mutants showed LR. Moreover, all the substitutions of amino acid point mutations including FfSdhBH248L/D/Y, FfSdhC2A83V,H144Y, and FfSdhDS106F,E166K conferring resistance to Pyd in F. fujikuroi were verified by protoplast transformation. Additionally, a positive cross-resistance was detected between Pyd and another SDHI fungicide penflufen, while no cross-resistance was detected between Pyd and phenamacril, prochloraz, azoxystrobin, carbendazim, or fludioxonil. Although pathogenicity of the mutants was increased compared with that of the wild-type parental strains, the mycelial growth rate and spore production levels of the resistant mutants were significantly decreased (P < 0.05), indicating significant fitness cost of resistance to Pyd in F. fujikuroi. Taken together, the risk of resistance to Pyd in F. fujikuroi might be moderate, and appropriate precautions against resistance development in natural populations should be taken into account when Pyd is used for the control of RBD.