无细胞蛋白质合成
糖酵解
氧化磷酸化
化学
磷酸烯醇丙酮酸羧激酶
ATP合酶
细胞
生物化学
能量代谢
酶
生化工程
蛋白质生物合成
生物物理学
组合化学
生物
工程类
内分泌学
作者
Kara Calhoun,James R. Swartz
出处
期刊:Humana Press eBooks
[Humana Press]
日期:2007-01-01
卷期号:: 3-17
被引量:57
标识
DOI:10.1007/978-1-59745-388-2_1
摘要
Supplying energy for cell-free protein synthesis reactions is one of the biggest challenges to the success of these systems. Oftentimes, short reaction duration is attributed to an unstable energy source. Traditional cell-free reactions use a compound with a high-energy phosphate bond, such as phosphoenolpyruvate, to generate the ATP required to drive transcription and translation. However, recent work has led to better understanding and activation of the complex metabolism that can occur during cell-free reactions. We are now able to generate ATP using energy sources that are less expensive and more stable. These energy sources generally involve multistep enzymatic reactions or recreate entire energy-generating pathways, such as glycolysis and oxidative phosphorylation. We describe the various types of energy sources used in cell-free reactions, give examples of the major classes, and demonstrate protocols for successful use of three recently developed energy systems: PANOxSP, cytomim, and glucose.
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