荧光素酶
抄写(语言学)
生物搬运器
报告基因
基因
DNA
转录调控
调节顺序
编码区
信使核糖核酸
基因表达调控
生物
分子生物学
遗传学
基因表达
转染
语言学
哲学
出处
期刊:Humana Press eBooks
[Humana Press]
日期:2003-11-15
卷期号:: 165-174
被引量:28
标识
DOI:10.1385/1-59259-686-x:165
摘要
AbstractReporter genes provide easy and efficient methods for the indirect measurement of relative rates of transcription. Utilizing common DNA cloning methods, a putative regulatory region can be coupled to the coding sequence of a reporter gene such that expression of the reporter protein product varies according to the regulatory potential of the DNA tested. The assays for reporter enzymes have the advantage of high sensitivity with low background, and, although an indirect measure, the amount of protein product is usually directly proportional to the level of transcriptional activation. Alternatives to reporter gene assays such as the direct measurement of the level of specific mRNAs for the endogenous gene can be influenced by RNA stability changes as well as transcription rates in response to stimulation. Assays for mRNA are also more labor intensive and difficult to quantify.KeywordsLuciferase ActivityPotassium Phosphate BufferTransfection EfficiencyReporter ConstructReporter SystemThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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