DNA甲基化
亚硫酸氢盐测序
照明菌甲基化试验
计算生物学
甲基化DNA免疫沉淀
表观遗传学
快照(计算机存储)
甲基化
生物
DNA
分子生物学
遗传学
基因
计算机科学
基因表达
数据库
作者
Zachary Kaminsky,Artūras Petronis
出处
期刊:Methods in molecular biology
日期:2009-01-01
卷期号:: 241-255
被引量:29
标识
DOI:10.1007/978-1-59745-522-0_18
摘要
As the role for epigenetic signals in genome regulation becomes increasingly understood, the ability to accurately measure levels of DNA methylation at individual cytosines throughout the genome is becoming increasingly important. In contrast to traditional methods for the quantification of cytosine methylation, such as cloning and sequencing of PCR fragments amplified from sodium bisulfite-modified DNA, recent developments have created a fast and effective alternative called methylation-sensitive single nucleotide primer extension (Ms-SNuPE). The following protocol outlines the steps necessary to design and perform Ms-SNuPE experiments using the SNaPshot chemistry and associated capillary electrophoresis platforms available through Applied Biosystems.
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