血脑屏障
蛛网膜下腔出血
骨桥蛋白
穿孔
免疫印迹
埃文斯蓝
药理学
医学
重组DNA
内科学
内分泌学
化学
中枢神经系统
生物化学
基因
材料科学
冲孔
冶金
作者
Hidenori Suzuki,Yu Hasegawa,Robert Ayer,Takashi Sugawara,Wanqiu Chen,Takumi Sozen,Kenji Kanamaru,Waro Taki,John H. Zhang
出处
期刊:Acta neurochirurgica
日期:2011-01-01
卷期号:: 231-236
被引量:13
标识
DOI:10.1007/978-3-7091-0693-8_39
摘要
Objects: We determined effects of recombinant OPN (r-OPN), a pleiotropic extracellular matrix protein, on blood-brain barrier (BBB) disruption and matrix metalloproteinase (MMP)-9 activation after subarachnoid hemorrhage (SAH) in rats. Methods: The endovascular perforation model of SAH was used. SAH or sham-operated rats were treated with pre-SAH intracerebroventricular administration of two dosages of r-OPN, r-OPN + GRGDSP (an L-arginyl-glycyl-L-aspartate-dependent integrin receptor antagonist), albumin or vehicle. Neurological impairments, brain edema and BBB disruption were evaluated, and Western blot analyses were performed in the brain at 24 h after SAH. Results: r-OPN significantly prevented brain edema and BBB disruption compared with the control rats, associated with the suppression of nuclear factor-κB and mitogen-activated protein kinase pathways, leading to MMP-9 inactivation. These effects were blocked by GRGDSP. Conclusions: L-arginyl-glycyl-L-aspartate-dependent integrin receptor-mediated multiple signaling pathways may be involved in the protective effects of r-OPN against BBB disruption after SAH.
科研通智能强力驱动
Strongly Powered by AbleSci AI