25‐Hydroxycholesterol metabolism is altered by lung inflammation, and its local administration modulates lung inflammation in mice

炎症 医学 新陈代谢 内科学
作者
Pauline Bottemanne,Adrien Paquot,Hafsa Ameraoui,Owein Guillemot‐Legris,Mireille Alhouayek,Giulio G. Muccioli
出处
期刊:The FASEB Journal [Wiley]
卷期号:35 (4) 被引量:21
标识
DOI:10.1096/fj.202002555r
摘要

Abstract Inflammation is a critical component of many lung diseases including asthma and acute lung injury (ALI). Using high‐performance liquid chromatography–mass spectrometry, we quantified the levels of oxysterols in two different murine models of lung diseases. These are lipid mediators derived from cholesterol and known to modulate immunity and inflammation. Interestingly, 25‐hydroxycholesterol (25‐OHC) was the only oxysterol with altered levels during lung inflammation, and its levels were differently affected according to the model. Therefore, we sought to assess how this oxysterol would affect lung inflammatory responses. In a model of lipopolysaccharide (LPS)‐induced acute lung inflammation, 25‐OHC levels were increased, and most of the hallmarks of the model (eg, leukocyte recruitment, mRNA expression, and secretion of inflammatory cytokines) were decreased following its intratracheal administration. We also found that, when administered in the lung, 25‐OHC is metabolized locally into 25‐hydroxycholesterol‐3‐sulfate and 7α,25‐dihydroxycholesterol. Their administration in the lungs did not recapitulate all the effects of 25‐OHC. Conversely, in a model of allergic asthma induced by intranasal administration of house dust mites (HDM), 25‐OHC levels were decreased, and when intranasally administered, this oxysterol worsened the hallmarks of the model (eg, leukocyte recruitment, tissue remodeling [epithelium thickening and peribranchial fibrosis], and cytokine expression) and induced changes in leukotriene levels. Ex vivo, we found that 25‐OHC decreases LPS‐induced primary alveolar macrophage activation while having no effect on neutrophil activation. Its sulfated metabolite, 25‐hydroxycholesterol‐3‐sulfate, decreased neutrophil, but not macrophage activation. Taken together, our data support a differential role of 25‐OHC in ALI and allergic inflammation models.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
畅快盼望发布了新的文献求助10
刚刚
anti1988完成签到,获得积分10
1秒前
1秒前
Akim应助落寞灵安采纳,获得10
1秒前
可爱的函函应助猪猪hero采纳,获得10
2秒前
NoNoQ完成签到 ,获得积分10
2秒前
2秒前
jingwenli21发布了新的文献求助10
2秒前
无花果应助neil采纳,获得10
2秒前
2秒前
Suen完成签到 ,获得积分10
2秒前
niuniu顺利毕业完成签到 ,获得积分10
2秒前
淡淡易云发布了新的文献求助100
3秒前
3秒前
ijn关闭了ijn文献求助
3秒前
向东东发布了新的文献求助10
3秒前
hhh发布了新的文献求助10
3秒前
研友_VZG7GZ应助clair采纳,获得20
4秒前
4秒前
淡然冬灵发布了新的文献求助10
4秒前
cheng完成签到,获得积分10
4秒前
5秒前
FashionBoy应助LRR采纳,获得10
5秒前
研友_VZG7GZ应助sc采纳,获得10
5秒前
酷波er应助YY采纳,获得10
6秒前
6秒前
呆萌发布了新的文献求助10
6秒前
7秒前
7秒前
传奇3应助发嗲的飞机采纳,获得10
7秒前
8秒前
可爱的函函应助电池小匠采纳,获得10
8秒前
猪猪hero发布了新的文献求助10
8秒前
8秒前
8秒前
9秒前
樱悼柳雪发布了新的文献求助10
10秒前
10秒前
11秒前
fffff发布了新的文献求助10
11秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3974602
求助须知:如何正确求助?哪些是违规求助? 3519026
关于积分的说明 11196787
捐赠科研通 3255127
什么是DOI,文献DOI怎么找? 1797693
邀请新用户注册赠送积分活动 877100
科研通“疑难数据库(出版商)”最低求助积分说明 806130