Assisted Reproductive Technologies (ARTs) in the Laboratory Mouse

Biomedical research has been hugely enriched by the advent of genetically engineered mouse models (GEMM). The latest genome editing technologies have enabled the development of numerous targeted genome-edited animals. GEMMs are valuable assets to the researchers for studying the pathophysiology of several human diseases. For the development of such animal models, assisted reproductive technologies (ARTs) in the laboratories play a very crucial role. ART helps in the preservation of valuable strains and safeguards them from infectious disease outbreaks and natural calamities. This also helps in the global distribution of strains to fulfill the research demands. ARTs involve superovulation, in vitro fertilization, cryopreservation of germplasms, and embryo transfer. The principles and practical aspects of ARTs are described.